中国激光, 2003, 30 (4): 369, 网络出版: 2006-06-27
532 nm激光激励血液中红细胞产生 荧光的物理机理研究
Analyzing Physical Mechanism about 532 nm Laser-induced Erythrocyte Fluorescence
血液生理学 荧光光谱 532 nm激光 红细胞 偶极非谐振子 blood physiology fluorescence spectrum 532 nm-laser erythrocyte non-syntonic dipole
摘要
通过建立偶极非谐振子模型对绿色激光激励红细胞产生荧光光谱的物理机理进行了研究。理论研究结果表明,波长为532 nm的低强度半导体激光抽运Nd:YAG倍频激光照射血液中的红细胞时,激光将使红细胞上不对称的C-C键或C-N键发生非谐振吸收而断裂,形成未成对电子,由于该种电子的产生而形成的新的荧光团在后续激光的作用下受激跃迁而产生荧光。通过研究上述绿色激光诱导红细胞产生的荧光反应,可能对解释低强度激光在医疗上的应用机理有一定的参考意义。
Abstract
The physical mechanism of green laser inducing erythrocyte fluorescence spectra in vitro is analyzed by a model of non syntonic dipole in this paper. The theoretical investigation shows that while the erythrocyte from blood is radiated by a low lever frequency doubled Nd∶YAG laser with wavelength 532 nm, anisomerous C-C bonds or C-N bonds on membrane of the erythrocyte would absorb the energy by non resonance and be parted to produce lone pairs of electrons. The new fluorophores from the lone pairs of electrons under the laser excitation would emit fluorescence. It may supply some references to the explanation the mechanism of low level laser irradiating blood therapy in vivo.
高淑梅, 孔艳, 沈中华, 陆建, 倪晓武. 532 nm激光激励血液中红细胞产生 荧光的物理机理研究[J]. 中国激光, 2003, 30(4): 369. 高淑梅, 孔艳, 沈中华, 陆建, 倪晓武. Analyzing Physical Mechanism about 532 nm Laser-induced Erythrocyte Fluorescence[J]. Chinese Journal of Lasers, 2003, 30(4): 369.