以动物血液为样品研究低强度激光对红细胞流变学特性的影响。将放置后的猪血血液(红细胞变形能力已变差)施以激光照射,测量红细胞变形能力的变化。用650 nm激光照射20 min后,红细胞变形能力有明显改善,此变形能力的改善随照射功率的增大而增强,至4～5 mW后趋于饱和。在相同的照射功率下(10 mW)650 nm与632.8 nm对红细胞变形能力的改善有相近的效应,这可由红细胞中血红蛋白对两波长的激光有相近的吸收得到解释。以小鼠血液为样品,研究激光照射对红细胞电泳率的影响。经632.8 nm激光照射后,红细胞的电泳率明显增加,此红细胞带电量的增加将有助于改善红细胞的聚集性。在所使用的激光功率下(小于20 mW),经形态学显微测量表明,红细胞并未造成可观察到的伤害,也未有溶血现象发生。

Using the blood samples of animal, the in vitro effects of low power laser irradiation on erythrocyte rheology were investigated. After the deposited pig′s erythrocytes (the deformation of erythrocytes have already turned to worse) were irradiated with laser, the effects of laser irradiation on erythrocyte′s deformation were measured. When 650 nm laser was used for irradiation, the deformability of erythrocytes was improved obviously. The changing tendency is that, the erythrocyte′s deformation was enhanced with the increasing of irradiation power and then saturated around 4～5 mW. When the blood samples were irradiated by 650 nm and 632.8 nm lasers respectively with the same power (10 mW), the erythrocyte deformability in two cases were all obviously increased to the similar level. These phenomena can be explained by that, the hemoglobin in erythrocytes has the similar absorption to both lasers. Taking the mouse blood as the samples, the effects of laser irradiation on erthrocyte′s electrophoretic mobility was studied further. When irradiated by 632.8 nm laser, the electrophoretic mobility of erythrocytes was speeded also, reflecting the electric charges on the surface of erythrocytes were increased which will help to decrease the aggregation of erythrocytes. Under such low power irradiation (less than 20 mW), no morphological change was detected by the microscopy and no hemolysis was found also.