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GFP标记的肿瘤生长和转移的整体荧光成像

Whole-body Fluorescent Imaging of the Growth and Metastasis of GFP-expressing Tumors

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摘要

Fugene 6脂质体介导pEGFP-C1转染人源肺癌细胞(SPC-A1),经G418抗性筛选和96孔板有限稀释获得稳定高表达GFP的单克隆细胞株SPC-A1-EGFP.裸鼠腹腔注射SPC-A1-EGFP细胞建立自发转移模型;裸鼠尾静脉注射SPC-A1-EGFP细胞建立实验转移模型.利用整体光学成像系统(whole-body optical imaging system)对荷瘤鼠整体荧光成像.结果表明,整体光学成像系统可实时非侵入监测腹腔肿瘤生长和扩散过程,通过胸腔皮瓣窗(chest-wall skin-flap window)可低侵入检测肺转移.该研究为在体监测原位移植瘤的自发转移和发现抗肿瘤新药物提供了良好实验平台.

Abstract

Human lung carcinoma cells(SPC-A1)were transfected with pEGFP-C1 by FuGENE 6 reagent, and screened by the culture medium contained geneticin (G418) and limiting dilution in 96-well flat-bottomed culture plates. A stable high GFP-expressing SPC-A1 cell line (SPC-A1-EGFP) was obtained and transplanted to the nude mice for preparing the tumor bearing mice. Two types of tumor metastasis models were prepared, spontaneous metastases by subcutaneous injection and experimental metastases by tail vein injection of SPC-A1-EGFP cells. The fluorescent signal of the tumor-bearing mice were detected using the whole-body optical imaging system. The results showed that the growth and metastasis of intraperitoneal tumors were visualized clearly with this system, while lung metastases were imaged through a chest-wall skin-flap window. This study provides a platform for monitoring the tumor metastasis by orthotopic implantation and evaluating

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中图分类号:Q63

所属栏目:激光生物医学

基金项目:教育部科学技术研究重大项目(10420);国家自然科学基金(60278017).

收稿日期:2004-07-05

修改稿日期:--

网络出版日期:2004-12-01

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储军:华中科技大学生物医学光子学教育部重点实验室,中国湖北,武汉,430074
陈延平:华中科技大学生物医学光子学教育部重点实验室,中国湖北,武汉,430074
熊涛:华中科技大学生物医学光子学教育部重点实验室,中国湖北,武汉,430074
张智红:华中科技大学生物医学光子学教育部重点实验室,中国湖北,武汉,430074
余雳:华中科技大学生物医学光子学教育部重点实验室,中国湖北,武汉,430074
骆清铭:华中科技大学生物医学光子学教育部重点实验室,中国湖北,武汉,430074

备注:骆清铭,通讯作者:027-7792033, E-mail: qluo@mail.hust.edu.cn.储军:男,1978年生,华中科技大学生物医学光子学教育部重点实验室2001级硕士,主要从事肿瘤的在体光学成像.

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引用该论文

储军,陈延平,熊涛,张智红,余雳,骆清铭. Whole-body Fluorescent Imaging of the Growth and Metastasis of GFP-expressing Tumors[J]. Acta Laser Biology Sinica, 2004, 13(6): 433-438

储军,陈延平,熊涛,张智红,余雳,骆清铭. GFP标记的肿瘤生长和转移的整体荧光成像[J]. 激光生物学报, 2004, 13(6): 433-438

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