为了了解生物组织不同结构对光透明进程的影响,利用光相干断层成像术(OCT)和近红外光谱(NIR)对肌肉组织(猪骨骼肌)和上皮组织(猪胃黏膜)经丙三醇处理后的光透明效果进行了比较研究。结果表明,这两种组织的光相干断层成像术成像深度和内层次结构的成像对比度均得到改善;肌肉组织和胃黏膜组织的光透射率分别增加了21%和16%,而漫反射率分别降低了33%和21%;这两种结构组织的光透明效果(肌肉组织较好)和进程(肌肉组织较快)与各自的失水率和失水动力学成正相关,肌肉组织失水过程为先快后慢;胃黏膜组织的失水过程为先慢后快;肌肉组织的总失水率大于胃黏膜组织。这表明结构不同的生物组织的光透明效果存在差异,必须考虑其内部结构的影响。

The dependence of optical clearing on properties of the different tissue structures is investigated in order to better know the clearing mechanisms and effective clearing approach. Two typical tissues, muscle and epithelial, have been chosen as the targets and the effect of optical clearing has been comparatively studied by optical coherence tomography (OCT) and near infrared spectroscopy (NIR) with the clearing agent glycerol used. The results show that the improved imaging depth and contrast among different layers in the porcine skeletal muscle and stomach mucosa are visualised with the OCT assessment. The overall increases in light transmittance in the muscle tissue and the stomach mucosal tissue after 30 min treatment are observed to be approximate 21% and 16%, decreases in diffuse reflectance are 33% and 21%, respectively, with the quantitative measurement of NIR. The clearing progress of each tissue type as a function of time corresponds very well with the respective loss in water dynamics shown by the near infrared spectra: early rapid transport, mid-linear and slow exponential diffusion for muscle tissue, and contrarily for stomach mucosa with an early very slow transport and later linear increasing efflux. The different permeabilities of muscle tissue and mucosal tissue are thought to be accountable for the difference in clearing effect between the two tissues. These observations confirm expectation that optical clearing is dependent on tissue structure in an explainable manner.