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Research on optical biosensor with up-converting phosphor marker

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Abstract

An optical biosensor with up-converting phosphor (UCP) marker is developed for the sensitive rapid immunoassay to the specific biomolecule. UCP can emit visible light when excited by infrared light. Through detecting and analyzing the content of UCP particles on the test strip after immunoreaction, the concentration of target analyte in the sample can be obtained. The detection sensitivity to plague IgG is better than 5 ng/ml; to plague FI-Ab is better than 100 pg/ml; to plague Yersinia pestis cell is better than 3*10^(4) CFU/ml. Good linear response characteristics and an excellent correlation (R2>=0.95) have been verified by quantitative detection results. In the practical application, detection results to 167 analytic samples have an excellent consistency with those obtained by reverse hemagglutination test. The up-converting phosphor technology (UPT) based biosensor has stable, reliable, and sensitive performances. It can meet the need of various bioassay applications.

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收稿日期:2006-03-20

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Yongkai Zhao:Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071
Lei Zhou:Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071
Jing Wang:Chinese Academy of Inspection and Quarantine, Beijing 100025
Lihua Huang:Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800
Zhongqiang Yan:Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071
Huijie Huang:Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800
Ruifu Yang:Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071
Lei Liu:Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800
Bingqiang Ren:Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800
Xiangzhao Wang:Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800

联系人作者:Yongkai Zhao(huanghuijie@siom.ac.cn)

备注:This work was supported by the National Natural Science Foundation of China under Grant No. 60378024. H. Huang is the author to whom the correspondence should be addressed, his e-mail address is huanghuijie@siom.ac.cn. Y. Zhao's e-mail address is yz107@hw.ac.uk

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引用该论文

Yongkai Zhao, Lei Zhou, Jing Wang, Lihua Huang, Zhongqiang Yan, Huijie Huang, Ruifu Yang, Lei Liu, Bingqiang Ren, Xiangzhao Wang, "Research on optical biosensor with up-converting phosphor marker," Chinese Optics Letters 4(8), 464-466 (2006)

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