从激光在厚试样中入射和受激发荧光在试样中出射时光能的吸收与散射损耗的基本原理获得了光学系统接收荧光的归一化光强表达式。给出了迭代法、FFT方法的数学模型,进行了计算模拟,并分析比较了FFT方法和迭代法的计算量和计算精度。给出了用FFT法层析恢复后的图像。

According to the principle of the light attenuation due to absorption and scattering of both the excitation and the fluorescence lights in a deep sectional thick sample, the normalized fluorescence intensity expression is obtained for the light received by an optical system. The mathematical models of the iterative and FFT treatment methods are given. Using computer analog the FFT method is compared with the iterative one in two respects of numerical accuracy and computational efficiencies. Finally, the real image after restoration of the sectional sample is reconstructed by computer simulation with the FFT method.