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Methods of calibration to optical trapping force upon non-spherical cells

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Abstract

The dynamical equation of a trapping cell is solved to find calibration methods for the trapping force, and two methods are compared by synthetic experiment data. Results indicate that: Boltzmann distribution method (BDM) is available for the force calibration of non-spherical or anisotropic cells in arbitrary trap potential; the mean square displacement method (MSDM) is available only for a symmetric harmonic optical trap. The spatial resolution requirement of the calibration system is about a nanometer. The results agree with the reported experiments.

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收稿日期:2005-11-23

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喻有理:The Key Laboratory of Biomedical Information Engineering of Ministry of Education, and Institute of Biomedical Engineering, Xi'an Jiaotong University, Xi'an 710049School of Science, Xi'an Jiaotong University, Xi'an 710049
张镇西:The Key Laboratory of Biomedical Information Engineering of Ministry of Education, and Institute of Biomedical Engineering, Xi'an Jiaotong University, Xi'an 710049
李政:The Key Laboratory of Biomedical Information Engineering of Ministry of Education, and Institute of Biomedical Engineering, Xi'an Jiaotong University, Xi'an 710049
王小力:School of Science, Xi'an Jiaotong University, Xi'an 710049

备注:This work was supported by the National Natural Science Foundation of China under Grant No. 60378018 and 60578026. Z. Zhang is the author to whom the correspondence should be addressed, his e-mail address is zxzhang@mail.xjtu.edu.cn. Y. Yu's e-mail address is youliyu@mail.xjtu.edu.cn

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引用该论文

喻有理, 张镇西, 李政, 王小力, "Methods of calibration to optical trapping force upon non-spherical cells," Chinese Optics Letters 4(12), 722-724 (2006)

被引情况

【1】王亚伟,韩广才,刘莹,程晓农,Frank Wyrowski. 血液细胞光相位分布特征的虚拟仿真研究. 中国激光, 2009, 36(6): 1595-1600

【2】李吉祥,喻有理,张孝林. 热驱动法测量光阱刚度的蒙特卡罗模拟. 光学学报, 2010, 30(1): 175-179

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