光子学报, 2006, 35 (11): 1709, 网络出版: 2010-06-03
二次谐波共焦成像的分辨率
Resolution of Confocal Scanning Microscope for Second-harmonic Wave
摘要
通过与双光子荧光共焦成像过程比较,研究了非线性二次谐波的强度脉冲响应函数并对它进行频域分析,获得了横向、纵向截止频率和通频带.研究结果表明:二次谐波共焦成像具有更高的分辨率.
Abstract
The three-dimensional intensity pulse function of a confocal scanning microscope for secondharmonic wave is derived and compared with that of a two-photon fluorescent confocal scanning microscope. By use of Fourier transformation,the three-dimensional intensity pulse function is theoretically investigated in frequency domain where the transverse and axial cut-off spatial frequencies,together with the transmission bands are given. The results show the optical resolution for second-harmonic wave is better than that for two-photon fluorescence. Such conclusion is also proved from the uncertainty principle.
杨初平, 旷卫民, 刘军, 唐志列. 二次谐波共焦成像的分辨率[J]. 光子学报, 2006, 35(11): 1709. Yang Chuping, Kuang Weimin, Liu Jun, Tang Zhilie. Resolution of Confocal Scanning Microscope for Second-harmonic Wave[J]. ACTA PHOTONICA SINICA, 2006, 35(11): 1709.