The depolarization behavior of backscattered linearly polarized light from ZnO thin film was investigated experimentally. The results show that the characteristics are related to both the polarization orientation and wavelength of linearly polarized incident light. When the incident light is s-polarized, the depolarization behaviors are different for different wavelengths. When the incident light is p-polarized, the depolarization behaviors, on the contrary, are similar for different wavelengths. In addition, there is an optimal incident angle for depolarization of linearly polarized light with different wavelengths, which is equal to their effective Brewster angles, respectively.