激光与光电子学进展, 2014, 51 (8): 082801, 网络出版: 2014-07-30
无标记快速检测蝇毒磷的表面等离子体共振生物传感器
Surface Plasmon Resonance Biosensor for Rapid Unmarked Coumaphos Detection
表面光学 生物医学信息技术 表面等离子体共振生物传感器 便携式 无标记快速检测 蝇毒磷 optics at surfaces biomedical information technology surface plasmon resonance biosensor portable unmarked rapid detection coumaphos
摘要
针对现有农药检测方法操作复杂、仪器贵重、不利于现场快速检测等问题,将自行研制的便携式表面等离子体共振生物传感器应用于高毒性农药蝇毒磷的检测,采用直接法检测抗体。提出连续检测法,对质量浓度分别为500、200、100、50、0 mg/L 的蝇毒磷小分子样品进行了连续检测实验。通过实验证明了该装置和方法的可行性。基于表面等离子体共振的无标记蝇毒磷检测方法简单,仪器便携,操作简便,可实现现场快速检测。
Abstract
The self-developed portable surface plasmon resonance biosensor is used in the detection of highly toxic pesticide coumaphos, as the existing pesticide detection methods are complicated, with precious instrument, and not conductive to on-site rapid detection. The antibody direct detection experiments are conducted; the continuous detection method is proposed and preceded in small coumaphos molecules with concentrations of 500, 200, 100, 50 and 0 mg/L. The validity of this method and the device is demonstrated by experiments. The unmarked coumaphos detection method based on the surface plasmon resonance is simple. The developed device is portable and easy to operate. The rapid and real-time detection can be realized by this method.
齐攀, 马骁, 钟金钢, 李莹. 无标记快速检测蝇毒磷的表面等离子体共振生物传感器[J]. 激光与光电子学进展, 2014, 51(8): 082801. Qi Pan, Ma Xiao, Zhong Jingang, Li Ying. Surface Plasmon Resonance Biosensor for Rapid Unmarked Coumaphos Detection[J]. Laser & Optoelectronics Progress, 2014, 51(8): 082801.