利用飞秒激光脉冲在细胞内部的非线性吸收，产生等离子体，通过产生等离子体诱导蚀除效应，从而达到细胞微手术的效果。对飞秒激光神经细胞突起切割的机理进行了初步分析，并研究了实验参量对切割作用和细胞活性的影响，发现平均功率≥0.5 mW的飞秒激光经40倍物镜(NA=0.60)聚焦进细胞中会产生光致破裂现象，导致作用细胞的死亡；稍低激光功率下在突起离胞体远近不同处切割，细胞均不会发生死亡，且稍远距离下切割细胞的突起似对细胞影响不大；不完全切割突起情况下，切割长度较小时，对细胞影响不大。

Femtosecond laser can induce nonlinear absorption in living cells, produce plasma, and achieve plasma-induced ablation and micro-surgery on cells. Dissection mechanism and influence of experiment parameters on dissection effect and cells′ activity are analyzed. The result shows that 1 kHz femtosecond laser with powers over 0.5 mW focused by 40× objective(NA=0.60) can induce photodisruption phenomena and kill target cell. Cells will survive with different dissection points as the laser power is below 0.4 mW. With incomplete dissection of short length, target cell changes little.