大气与环境光学学报, 2008, 3 (1): 0036, 网络出版: 2010-05-21
LED激发光源的水体浮游植物浓度活体检测系统研究
Research on In-vivo Measurement of Phytoplankton Biomass by LED Excitation Source
摘要
采用5种不同中心激发波长的超高亮LED作为激发光源构建了一套水体浮游植物活体荧光检测系统。利用该系统测量24种不同来源水样和同一水样10种不同比例稀释样品的浮游植物活体荧光强度,研究了荧光强度与叶绿素a浓度的线性关系。结果表明:蓝光LED(最大发射波长442.7 nm)是用以测量浮游植物活体荧光的最适合光源,相应的检测限达到了0.0278 μg/l(叶绿素a);浮游植物种类、环境造成的荧光量子效率以及水体DOM背景荧光均对活体荧光的测量结果产生影响,尤以浮游植物种类和荧光量子效率的影响更为显著。因此,蓝光超高亮LED将为浮游植物活体荧光测量提供廉价的替代光源,为利用该光源研发浮游植物浓度原位荧光检测仪提供了潜在的可能。为克服浮游植物种类、荧光量子效率以及DOM背景荧光对浮游植物活体荧光测量结果的影响,需要进一步研究上述因素的具体影响行为,发展相应方法进行校正。
Abstract
An experiment system for determining in vivo fluorescence of phytoplankton with five LEDs with different maximum emission wavelengths as excitation light sources was established. Based on the system,in vivo fluorescence of 10 different dilutions from the same water sample and 24 kinds of water samples from different sources was determined and the linear relationship of fluorescence intensity and chlorophylla concentration was also investigated. The results indicate that blue LED(maximum emission wavelength 442.7 nm) is the most proper excitation light source for determining in vivo fluorescence of phytoplankton,and limit of determination is 0.0278 μg/1(chlorophyll-a). Background fluorescence of DOM, especially the difference of phytoplankton species and fluorescence quantum efficiency, shows significant influence on the determination of in vivo fluorescence. Blue LED has a potential as excitation light source of phytoplankton fluorometry. In order to overcome the influence of phytoplankton species, fluorescence quantum efficient and DOM background fluorescence on in vivo fluorescence, further research should be carried out and proper adjustment methods are needed.
王志刚, 刘文清, 张玉钧, 司马伟昌, 赵南京, 肖雪, 刘建国. LED激发光源的水体浮游植物浓度活体检测系统研究[J]. 大气与环境光学学报, 2008, 3(1): 0036. WANG Zhi-gang, LIU Wen-qing, ZHANG Yu-jun, SIMA Wei-chang, ZHAO Nan-jing, XIAO Xue, LIU Jian-guo. Research on In-vivo Measurement of Phytoplankton Biomass by LED Excitation Source[J]. Journal of Atmospheric and Environmental Optics, 2008, 3(1): 0036.