Chinese Optics Letters, 2008, 6 (12): 938, Published Online: Dec. 17, 2008
Micro-Raman spectroscopy of single leukemic cells Download: 505次
300.6450 Spectroscopy, Raman 170.0170 Medical optics and biotechnology 170.1530 Cell analysis 170.1790 Confocal microscopy
Abstract
The Raman spectra from leukemic cell line (HL60) and normal human peripheral blood mononuclear cells (PBMCs) are obtained by confocal micro-Raman spectroscopy using near-infrared laser (785 nm) excitation. The scanning range is from 500 to 2000 cm^{-1}. The two average Raman spectra of normal PBMCs and carcinoma cells have clear differences because their structure and amount of nucleic acid, protein, and other major molecules are changed. The spectra are also compared and analyzed by principal component analysis (PCA) to demonstrate the two distinct clusters of normal and transformed cells. The sensitivity of this technique for identifying transformed cells is 100%.
Changmei Cai, Rong Chen, Juqiang Lin, Yongzeng Li, Shangyuan Feng. Micro-Raman spectroscopy of single leukemic cells[J]. Chinese Optics Letters, 2008, 6(12): 938.