Chinese Optics Letters, 2008, 6 (12): 941, Published Online: Dec. 17, 2008
A feasible method for comparing the power dependent photostability of fluorescent proteins Download: 630次
荧光蛋白 光稳定性 荧光显微成像 160.2540 Fluorescent and luminescent materials 180.2520 Fluorescence microscopy 300.6390 Spectroscopy, molecular
Abstract
A feasible method of combining the concept of fluorescence half-life and the power dependent photobleaching rate for characterizing the practical photostability of fluorescent proteins (FPs) was introduced. Furthermore, by using a fluorescent photostability standard, a relative comparison of the photostabilty of FPs from different research groups was proposed, which would be of great benefit for developing novel FPs with optimized emission wavelength, better brightness, and improved photostability. We used rhodamine B as an example to verify this method and evaluate the practical photostability of a far-red FP, mKate-S158C. Experimental results indicated good potential of this method for further study.
Jun Liu, Jun Chu, Hongyu Zhu, Lingling Xu, Zhihong Zhang, Shaoqun Zeng, Zhenli Huang. A feasible method for comparing the power dependent photostability of fluorescent proteins[J]. Chinese Optics Letters, 2008, 6(12): 941.