基于目前国内对于细胞工厂（1~40层培养皿）监测观察方法的局限性，为了方便观察，节省时间，提高工作效率，设计出一款用于监测“细胞工厂”中培养细胞的长工作距离的视频显微物镜。设计利用卡塞格林反射系统，放大倍率为5×，工作距离85 mm，数值孔径(NA)达到0.3，根据培养皿中的细胞大小，经过计算，运用ZEMAX软件进行模拟，配以维视图像生产的MV-200UC 1/3″电荷耦合器件(CCD)进行观察，接以视频显示器二次放大，总放大倍率M在200倍左右，系统分辨的最小距离在1.1 μm左右，满足分辨培养皿细胞的要求。

Due to the limitation of observation method in cell factory (1~40 layer dishes) monitoring in China, this paper designs a long-working-distance video objective for observing the cultured cells in cell factory, to facilitate the observation, save time and improve efficiency. According to the size of cells, the high resolution video objective, with 5 times magnification and 85 mm working distance, is designed by applying ZEMAX stimulation and Cassegrain reflector system. Amplified again by the video display, the total magnification is about 200 times, the numerical aperture is up to 0.3 and the liner field object height y is 0.6 mm, equipped with MV-200UC 1/3″ charge couple device (CCD) produced by Microvision in observation. The minimum distance resolution of the system is about 1.1 μm, meeting the requirement of distinguishing petri dish cells.