对牛血清白蛋白(BSA)与盐酸特拉唑嗪(THD)混合体系进行了荧光发射光谱和同步光谱实验研究。BSA溶液在340 nm处有明显荧光特征峰, 当加入THD后导致牛血清白蛋白发生内源荧光猝灭。据此建立了以BSA为探针的THD含量测定方法, 通过该方法所得的模型函数相关系数都高于0.99。对所得模型函数进行了实验验证, 其中发射光谱模型函数回收率为96.04%~103.16%, 同步光谱模型函数回收率为100.41%~105.58%, 相对标准偏差分别为3.70%和2.42%。荧光发射光谱和同步光谱方法的检出限分别为0.64×10-8 mol/L和0.66×10-8 mol/L, 定量限分别为0.21×10-7 mol/L和0.22×10-7 mol/L。

The fluorescence emission and synchronous spectra of the bovine serum albumin (BSA) and terazosin hydrochloride (THD) mixed system were studied. The most intensive characteristic peak (340 nm) was found in the spectrum of BSA solution, and the addition of THD resulted in the fluorescence quenching of BSA. Based on this phenomenon, the determination method for THD was established, in which BSA was used as the fluorescent probe． The correlation coefficients of the model functions are all more than 0.99. The recoveries of emission and synchronous spectra are 96.04%~103.16% and 100.41%~105.58%, the relative standard deviations (RSD) are 3.70% and 2.42%, the limits of detection are 0.64×10-8 mol/L and 0.66×10-8 mol/L, and the limits of quantification are 0.21×10-7 mol/L and 0.22×10-7 mol/L, respectively.