为了研究癌细胞自体荧光光谱在细胞周期变化过程中是否发生变化,对同步化培养的宫颈癌细胞(HeLa)在细胞周期各时相(G1期, S期, G2期和M期)样品的自体荧光谱进行了测量。测量结果表明,HeLa细胞在细胞周期变化过程中有自体荧光现象存在, 其特征荧光峰位于360 nm和680 nm处, 且各时相样品的荧光光谱强度各不相同。这些差异说明HeLa细胞在细胞周期变化过程中细胞内荧光物质(含芳香族氨基酸和卟啉)产生了相应的变化从而导致了不同时相的荧光光谱强度的差异。细胞周期各时相的荧光光谱能够反映细胞生长变化过程中芳香族氨基酸和卟啉的变化, 可为采用光谱技术对癌细胞生长周期进行研究提供依据。

In order to study the autofluorescence spectral changes during cancer cell cycle, the autofluorescence spectrum of synchronically cultured HeLa cell in the G1, S, G2 and M portions of the cell cycle is measured. The results show that there is autofluorescence phenomenon during the cell cycle and the fluorescence intensity in each stage is different. The fluorescence sptectrum exhibits the characteristic peaks at 360 nm and 680 nm. The changes of fluorescent molecules (such as aromatic amino acid and porphyrin) in HeLa cells during the cell cycle result in these differences. The fluorescence spectra of different stages reflect the changes of aromatic amino acid and porphyrin in cell during the cell cycle and can be utilized to study the state of the cancer cell cycle.