光学学报, 2009, 29 (6): 1609, 网络出版: 2009-06-08
激光参数对纳米金靶向细胞膜通透性的影响
Influence of Laser Parameters on Permeability of Gold Nanaoparticles Targeting Cells
医用光学与生物技术 激光参数 通透性 纳米金颗粒 热效应 medical optics and bitechnology laser parameters permeability gold nanoparticle thermal effect
摘要
激光参数,比如脉宽、照射能量、脉冲数目以及照射方式等在各个方面尤其是在生物医学应用中对生物组织的不同效应都起着重要作用。采用纳米金颗粒靶向细胞, 激光照射后细胞膜的通透性会发生改变。采用不同的激光光源和不同的照射方式(直接照射和扫描照射)、调整不同的激光参数照射细胞微粒结合体, 通过流式细胞仪测量细胞对异硫氰酸荧光素葡聚糖 (fluorescein isothiocyanate-Dextran, FITC-D) 和碘化丙啶 (propidium iodide PI) 的吸收量来判断细胞膜的暂时通透性和细胞的死亡率, 用以研究激光参数变化对细胞膜通透性的影响。采用传热模型对其机理进行了分析, 结果表明通过调整激光参数, 采用这种方法有可能进行基因转染。
Abstract
In medicine and biology when the laser is used, the parameters, such as pulse duration, irradiation energy,pulse number and irradiation manner are very important. Light-absorbing nanoparticles that are heated by short laser pulses can transiently increase the membrane permeability. Different laser sources and irradiation manner were used to investigate the transfection efficiency accociated with the laser parameters. After irradiation, the memebrane permeability was evaluated by flow cytometry assaying of propidium iodide (PI) and fluorescein isothiocyanate (FITC) dextran. The mechanism of light-induced damage was studied by thermal modeling. The preliminary results suggest that this laser-induced permeability strategy provides a promising new means of delivering larger exogenous materials such as plasmid DNA into living cells by adjusting the laser parameter.
姚翠萍, 张镇西. 激光参数对纳米金靶向细胞膜通透性的影响[J]. 光学学报, 2009, 29(6): 1609. Yao Cuiping, Zhang Zhenxi. Influence of Laser Parameters on Permeability of Gold Nanaoparticles Targeting Cells[J]. Acta Optica Sinica, 2009, 29(6): 1609.