首页 > 论文 > 中国激光 > 36卷 > 10期(pp:2552-2556)

基于透射和反射时间分辨测量数据的荧光参数三维重建

Reconstructing Three-Dimensional Fluorescent Parameters Using Time-Resolved Data Based on Transmittance and Reflection Measurements

  • 摘要
  • 论文信息
  • 参考文献
  • 被引情况
  • PDF全文
分享:

摘要

提出了一种同时重建荧光参数的全三维时域荧光分子层析方法,通过选择一对合适的拉普拉斯变换因子提取时间扩展曲线的特征信息,求解时域耦合扩散方程。在图像重建过程中,应用代数重建技术求解线性逆问题,且引入归一化玻恩比方法。基于多通道时间相关单光子计数系统对模拟仿体进行了透射和反射式时间分辨数据测量,利用所提方法同时重建了荧光产率和寿命的三维图像。实验结果表明利用反射式测量数据目标体位置、大小、形状等方面具有一定的偏差,透射式达到了较好的重建效果,而两种模式相结合重建效果最优。

Abstract

We present a full three-dimensional algorithm for time-domain fluorescence molecular tomography. A pair of appropriate transform-factors is employed to extract the featured information on the temporal profiles for time-domain coupled diffusion equations. The linear inversions at two distinct transform-factors are solved with an algebraic reconstruction technique,and the normalized Born ratio is proposed. The simulate phantom is measured in reflection and transmittance modes by use of multi-channel time-correlation single photon counting system. We experimentally validate that the proposed scheme can achieve simultaneous three-dimensional reconstruction of the fluorescent yield and lifetime. The results show that for the positions,sizes and shapes of the targets,there are some deviation in reflection measurement,the quality in transmittance one is better and in the two-mode is more satisfied.

广告组1 - 空间光调制器+DMD
补充资料

中图分类号:Q63

DOI:10.3788/cjl20093610.2552

所属栏目:医学与生物成像

基金项目:国家863计划(2009AA02Z413)、国家自然科学基金(60578008)、天津市自然科学基金(07JCYBJC06600)和高等学校学科创新引智计划(B07014)资助课题。

收稿日期:2009-07-02

修改稿日期:2009-08-10

网络出版日期:0001-01-01

作者单位    点击查看

张丽敏:天津大学 精密仪器与光电子工程学院,天津 300072
高峰:天津大学 精密仪器与光电子工程学院,天津 300072
李娇:天津大学 精密仪器与光电子工程学院,天津 300072
赵会娟:天津大学 精密仪器与光电子工程学院,天津 300072

联系人作者:张丽敏(zhanglm@tju.edu.cn)

备注:张丽敏|讲师|主要从事生物医学光电成像方面的研究|(1980-),女,博士。

【1】S.R. Cherry. In vivo molecular and genomic imaging:new challenges for imaging physics[J]. Physics in Medicine and Biology,2004,49(3):13-48

【2】T. F. Massoud,S. S. Gambhir. Molecular imaging in living subjects:seeing fundamental biological processes in a new light[J]. Genes Development,2003,17(5):545-580

【3】V. Ntziachristos,C. Bremer,E. E. Graves et al.. In vivo tomographic imaging of near-infrared fluorescent probes[J]. Molecular Imaging,2002,1(2):82-88

【4】Mao Zhengle,Wang Chen,Cheng Ya. Superresolution Far-field fluorescence bio-imaging breaking the diffraction barrier[J]. Chinese J. Lasers,2008,35(9):1283-1307
毛峥乐,王琛,程亚. 超分辨远场生物荧光成像-突破光学衍射极限[J]. 中国激光,2008,35(9):1283-1307

【5】Qu Xiaochao,Liang Jiaming,Yao Cuiping et al.. Optical properties of gold nanoparticle and its application in biological imaging and photothermal therapy[J]. Chinese J. Lasers,2007,34(11):1459-1465
屈晓超,梁佳明,姚翠萍 等. 金纳米微粒的光学性质及其在生物成像和光热疗法中的应用[J]. 中国激光,2007,34(11):1459-1465

【6】V. Ntziachristos,C. H. Tung,C. Bremer et al.. Fluorescence molecular tomography resolves protease activity in vivo[J]. Nature Medicine,2002,8(7):757-760

【7】H. Jiang. Frequency-domain fluorescent diffusion tomography:a finite-element-based algorithm and simulation[J]. Appl. Opt.,1998,37(22):5337-5343

【8】A. B. Milstein,S. Oh,K.J. Webb et al.. Fluorescence optical diffusion tomography[J]. Appl. Opt.,2003,42(16):3081-3094

【9】A. X. Cong,G. Wang. A finite-element-based reconstruction method for 3D fluorescence tomography[J]. Opt. Express,2005,13(24):9847-9857

【10】E. M. Sevick-Muraca,J.P. Houston,M. Gurfinkel. Fluorescence-enhanced,near infrared diagnostic imaging with contrast agent[J]. Current Opinion in Chemical Biology,2002,6(5):642-650

【11】M. A. O’Leary,D. A. Boas,X. D. Li et al.. Fluorescence lifetime imaging in turbid media[J]. Opt. Lett.,1996,21(2):158-160

【12】A. T. N. Kumar,J. Skoch,B.J. Bacskai et al.. Fluorescence-liftetime-based tomography for turbid media[J]. Opt. Lett.,2005,30(24):3347-3349

【13】V. Y. Soloviev,K. B. Tahir,J. McGinty et al.. Fluorescence lifetime tomography by using time-gated data acquisition[J]. Appl. Opt.,2007,46(30):7384-7391

【14】V. Y. Soloviev,J. McGinty,K. B. Tahir et al.. Fluorescence lifetime tomography of live cells expressing enhanced green fluorescent protein embedded in a scattering medium exhibiting background autofluorescence[J]. Opt. Lett.,2007,32(14):2034-2036

【15】S. Lam,F. Lesage,X. Intes. Time domain fluorescent diffuse optical tomography:analytical expressions[J]. Opt. Express,2005,13(7):2263-2275

【16】Zhang Limin,He Huiyuan,Gao Feng et al.. Image reconstruction in fluorescence molecular tomography based on full time-resolved scheme[J]. Acta Optica Sinica,2008,28(7):1262-1268
张丽敏,和慧园,高峰 等. 荧光分子层析中的全时间分辨图像重建法[J]. 光学学报,2008,28(7):1262-1268

【17】F. Gao,H. J. Zhao,Y. Tanikawa et al.. A linear,featured-data scheme for image reconstruction in time-domain fluorescence molecular tomography[J]. Opt. Express,2006,32(14):7109-7124

【18】Zou Wei,Wang Jiajun,Feng Dagan. Parallel computation of forward problem for fluorescence molecular tomography[J]. Acta Optica Sinica,2007,27(3):443-450
邹玮,王加俊,冯大淦. 荧光分子断层成像正向问题的并行计算[J]. 光学学报,2007,27(3):443-450

【19】A. Soubret,J. Ripoll,V. Ntziachristos. Accuracy of fluorescent tomography in the presence of heterogeneities:study of the normalized Born ratio[J]. IEEE Trans. Med. Imaging,2005,24(10):1377-1386

【20】Limin Zhang,Feng Gao,Huiyuan He et al.. Three-dimensional scheme for time-domain fluorescence molecular tomography based on Laplace transforms with noise-robust factors[J]. Opt. Express,2008,16(10):7214-7213

【21】Feng Gao,Limin Zhang,Jiao Li et al.. Experimental validation for time-domain fluorescence diffuse optical tomography of linear scheme[J]. Chin. Opt. Lett.,2008,6(12):889-892

引用该论文

Zhang Limin,Gao Feng,Li Jiao,Zhao Huijuan. Reconstructing Three-Dimensional Fluorescent Parameters Using Time-Resolved Data Based on Transmittance and Reflection Measurements[J]. Chinese Journal of Lasers, 2009, 36(10): 2552-2556

张丽敏,高峰,李娇,赵会娟. 基于透射和反射时间分辨测量数据的荧光参数三维重建[J]. 中国激光, 2009, 36(10): 2552-2556

被引情况

【1】李娇,高峰,易茜,张丽敏,赵会娟. 基于二维圆域解析模型的时域扩散荧光层析原理与实验研究. 中国激光, 2010, 37(11): 2743-2748

【2】刘 敏,彭长德,刘一曼,施 建. 基于时域的时间分辨荧光光谱的蒙特卡洛模拟. 激光生物学报, 2010, 19(5): 575-579

【3】张雁琦,王欣,尹国艳,李娇,马文娟,周仲兴,赵会娟,高峰,张丽敏. 面向吲哚菁绿药代动力学成像的动态实验系统. 中国激光, 2017, 44(1): 107001--1

您的浏览器不支持PDF插件,请使用最新的(Chrome/Fire Fox等)浏览器.或者您还可以点击此处下载该论文PDF