提出了一种同时重建荧光参数的全三维时域荧光分子层析方法,通过选择一对合适的拉普拉斯变换因子提取时间扩展曲线的特征信息,求解时域耦合扩散方程。在图像重建过程中,应用代数重建技术求解线性逆问题,且引入归一化玻恩比方法。基于多通道时间相关单光子计数系统对模拟仿体进行了透射和反射式时间分辨数据测量,利用所提方法同时重建了荧光产率和寿命的三维图像。实验结果表明利用反射式测量数据目标体位置、大小、形状等方面具有一定的偏差,透射式达到了较好的重建效果,而两种模式相结合重建效果最优。

We present a full three-dimensional algorithm for time-domain fluorescence molecular tomography. A pair of appropriate transform-factors is employed to extract the featured information on the temporal profiles for time-domain coupled diffusion equations. The linear inversions at two distinct transform-factors are solved with an algebraic reconstruction technique,and the normalized Born ratio is proposed. The simulate phantom is measured in reflection and transmittance modes by use of multi-channel time-correlation single photon counting system. We experimentally validate that the proposed scheme can achieve simultaneous three-dimensional reconstruction of the fluorescent yield and lifetime. The results show that for the positions,sizes and shapes of the targets,there are some deviation in reflection measurement,the quality in transmittance one is better and in the two-mode is more satisfied.