为了简化生化分析过程,提高测量精度,提出了统一积分时间数据测量法。根据光电二极管阵列(PDA)驱动程序和光电转换原理,推导出光强与积分时间档的关系式;然后,在实验的基础上按照PDA性能参数确定线性工作区间,再将各个积分时间暗电流数据记录下来,在数据采集过程中将相应的积分时间档下的暗电流数据扣除,以保证暗电流给分析仪器造成的误差最小。实验结果表明,采用该方法使工作效率提高了1~3倍,检测精度提高了25%。在以光纤光谱仪为主体的微型生化分析仪样品检测中的应用表明,采用该方法简化了实验步骤,在测试样品浓度发生很大变化时,也可以使仪器有较高的测试精度。

With the aim to improve the performance of a biochemical analyzer,an uniform integral time method for CCD data acquisition was presented in the paper. Different integral time data were calculated based on a Photo Diode Array(PDA) driving program and the photoelectrical transformation principle,and the linearity region of the biochemical analyzer was confirmed according to both performance parameters and experiments. Then,different integral time dark currents were recorded in the experiments and they were removed from an original spectrum to ensure the analyzer error in minimum. The experimental results show that the precision of the analyzer has been improved by 25%,and the work efficiency is 1~3 times higher than those of the original methods. It is proved that the using the proposed method can simplify the experimental procedures and can provide the higher precision for the analyzer even in sample concentration to be changed greatly.