为了解决现有的微喷点技术中外接驱动设备过于庞大,MEMS技术制作的微喷头结构复杂,与生物样品的兼容性差等问题,提出了新型脉冲惯性力驱动方式和无热源、无外部加压装置的微喷点系统用于微流体数字化喷点技术。介绍了微流体数字化喷点技术的驱动原理,搭建了微喷点系统的实验平台;然后,实验研究了样品均一性和驱动参数对微喷点的影响,并对实验数据进行分析和总结;最后,用黏度为4.13 mPa·s的点样液制作了密度为4 000点/cm2的中等密度微阵列。实验结果表明:样品点直径＜100 μm,变异系数CV＜8%,完全可以满足微阵列制备技术的密度、样品点尺度和均一性的要求,可供小型实验室使用。

A digital dispensing technology by using a pulse inertial force drived micro dispensing system without a heat resource and a pneumatic actuator for microfluids was presented to slove the existing problems on the huge external driving device,complicated configuration manufactured by MEMS technology and poor operability and compatibility with biological samples.The principle of digital dispensing technology of microfluids was introduced.Then,effects of the sample uniformity and driven parameters on dispensing technology were researched and the experimental data were analysed.Finally, the medium-density microarray was accomplished with the low viscosity liquid at 4.13 mPa·s.The experimental results show that the average diameter of spots is below 100 μm,and the Coefficients of Variation (CV) for diameters of spots are clearly below the measurement error (8%).It can satisfy the microarray requirements for the density,uniformity and sizes of spots,and can be used in small laboratories.