中国激光, 2009, 36 (11): 3084, 网络出版: 2009-11-11   

激光照射对急性脊髓损伤后脊髓再生的促进作用

Positive Effect of Low Power Laser Irradiation on Neuron Regeneration after Acute Spinal Cord Injury
作者单位
1 第四军医大学西京骨科医院,陕西 西安 710032
2 第四军医大学神经生物研究所,陕西 西安 710032
摘要
建立大鼠皮质脊髓背侧束全横断模型,利用弱激光照射脊髓受损部位的皮肤,观察激光照射对急性脊髓损伤后脊髓再生的促进作用。30只SD大鼠,随机分成对照组和照射组。照射组:急性皮质脊髓背侧束全横断后15 min进行连续14 d采用弱激光照射脊髓受损部位的皮肤。对照组:急性皮质脊髓背侧束全横断后未行弱激光经皮照射治疗。术后两组分别于第3,7,14 d分别取材,用苏木精-伊红染色法(HE)染色和免疫荧光标记染色观察。实验发现,脊髓损伤后14 d,照射组的空洞及瘢痕形成面积小于对照组,有统计学差异(p<0.05);对照神经胶质酸性组蛋白(GFAP)和硫酸软骨素(CS)表达强烈分布紧密,照射组GFAP和CS56表达达微弱且分布稀疏;对照组少量神经微丝蛋白(NF)在损伤区周围,神经生长相关蛋白(GAP43)形态肿大变形分布紊乱,照射组大量成纤维丝状的NF分布在损伤区周围,并与GAP43相伴行。结果表明,脊髓损伤急性期采用弱激光照射脊髓受损部位的皮肤,可减少脊髓损伤后空洞形成,并促进轴突再生。
Abstract
This study designed to establish a complete transaction of dorsal corticospinal tract SD rat model and discuss about the effect of low power laser irradiation on neuron regeneration after acute spinal cord jury. 30 SD rats were equally and randomly divided into two groups. Complete transaction of dorsal corticospinal tract was done to each rat. Then,irradiation group was undertaken local consecutive low power laser irradiation 15min after surgery and lasted for 14 days. Compared to irradiation group,there was no postoperative irradiation after surgery in control group. After infusion,1 cm length of spinal cord,which was above and below T8 level,were extracted on the 3rd,7th and 14th day after surgery. Hematoxylin and eosin stain and immunofluorescence stain were use for evaluation. At two weeks after surgery,the value of gial scar area was less than that of control group with statistically significant differences. There was intensive expression of glial fibrillary acidic protein (GFAP) and Chondroitin Sulfate (CS56) around the injury area in control while sparsate distribution of GFAP and CS56 was found in irradiation group. An amount of fibriform Neurofilament (NF) expression associated with regularly Growth Associated Protein 43 (GAP43) expression were located around the injury area,However,scrambled and slight GAP43 and NF expression were found in control. The result of our study showed that low power laser irradiation can improve regenerate of axon and decrease formation of gial scar area during the acute stage of spinal cord injury.

王哲, 夏雷, 龚凯, 刘飞, 王健, 徐新智, 罗卓荆. 激光照射对急性脊髓损伤后脊髓再生的促进作用[J]. 中国激光, 2009, 36(11): 3084. Wang Zhe, Xia Lei, Gong Kai, Liu Fei, Wang Jian, Xu Xinzhi, Luo Zhuojing. Positive Effect of Low Power Laser Irradiation on Neuron Regeneration after Acute Spinal Cord Injury[J]. Chinese Journal of Lasers, 2009, 36(11): 3084.

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