对Goldview(GV)作为荧光标记物的DNA荧光毛细生物传感器进行了研究。以荧光毛细分析法(fluorescence capillary analysis,FCA)为基础,在毛细管内壁通过Poly-l-lysine将20-mer-ssDNA探针固定,制成DNA荧光毛细生物传感器(DNA fluorescence capillary biosensor,DNA-FCB),DNA-FCB与互补靶DNA杂交,通过GV染色后,检测杂交产物的荧光强度,实现对靶DNA的定性和定量分析。样品用量12 μL,靶DNA的浓度在0.4-4 μmol·L^-1 (2.4-24 mg·L^-1 )范围内和荧光强度有良好的线性关系(y=65.911x+3.994 4,r=0.998 9);RSD<3.5%,检出限0.39 μmol·L^-1 (2.2 mg·L^-1 ),能达到定量检测靶DNA的目的。用DNA-FCB测定靶DNA操作简便,试样、试剂用量少,测定成本极低,能大大减少环境污染。

Goldview marked DNA fluorescence capillary biosensor was studied in the present paper.Based on fluorescence capillary analysis (FCA),the DNA biosensor uses capillary as immobilization carrier and detection carrier of DNA probe.Probes (20-mer-ssDNA) were immobilized on the inner wall of capillary by poly-l-lysine,and DNA fluorescence capillary biosensor (DNA-FCB) was made.After being hybridized with complementary target DNA and dyed by Goldview,the target DNA was qualified or quantified by detecting the fluorescence density of thE_goldview using F-4500 spectrofluorometer.The sample volume was 12 μL.The concentration of the target DNA showed good linearity with the fluorescence intensity in the range of 0.4-4 μmol·L^-1 (2.4-24 mg·L^-1 )(y=65.911x+3.994 4,r=0.998 9).The RSD was lower than 3.5%.The concentration detection limit of the target DNA was 0.39 μmol·L^-1 (2.2 mg·L^-1 ).The DNA-FCB can be used to qualify or quantify the target DNA.It’s advantages are simplicity of manipulation,thimbleful of sample and reagent volumes,repeated use of capillary,and the lowest test cost.By using DNA-FCB to qualify the target DNA,we can consumedly decrease the pollution of the environment.