近红外光谱(NIRS)分析技术是一项高效、快捷检测样品中某种物质成分的新方法,近年来在饲料营养分析、品种鉴别等领域得到了广泛的应用。作者提出了一种用近红外指纹光谱快速鉴别紫花苜蓿品种耐盐性的新方法。首先用近红外吸收光谱对20个紫花苜蓿品种进行聚类,发现供试品种很好地聚为耐盐和敏盐两类,与常规检验结果基本相同,通过聚类建立苜蓿品种耐盐性鉴别模型,其次用所建耐盐品种评价鉴别模型对6个苜蓿品种进行耐盐性预测,品种识别准确率达到100％,表现指数为85.7％。所以近红外吸收指纹图谱对苜蓿品种耐盐性具有很好的分类和鉴别作用,通过建立鉴别模型,可对品种耐盐性进行快速、高效的初步鉴定。

Near infrared reflectance spectroscopy is a new developed method able to analyse the content of specific compound in the detected sample quickly and efficiently,and has been wildly used in many fields such as evaluation of nutrition value of forage,cultivar discrimination and so on.In the present paper a new method was developed for the discrimination of salt tolerance of alfalfa cultivars by Fourier transform near infrared reflectance spectroscopy.Firstly absorbance spectrum generated by Fourier transform near infrared spectrometry was used to classify twenty alfalfa cultivars.These cultivars were obviously clustered into two groups:salt tolerant and salt sensitive,similar to the result screened by the traditional methods.Based on these results,a discrimination model was built for identification of salt tolerant cultivars.And then,six other cultivars were used to verify the function of the discriminating model.Result showed that the distinguishing rate of 100% was achieved with the performance index of 85.7%.It is concluded that Fourier transform near infrared spectrum is useful for classification and discrimination of salt tolerance of alfalfa cultivars.The method can be applied to estimate salt tolerance of alfalfa cultivars,and works more quickly and efficiently than the traditional screening methods.Application of Fourier transform near infrared spectrum to discriminating salt tolerance of alfalfa cultivars is significant in both academic and technical areas.