通过对肿瘤细胞生长过程中的光谱变化进行研究,寻求反应其生长变化过程的光学检测方法。根据Beer-Lambert定律推知,在肿瘤细胞的生长变化过程中,细胞内的蛋白质、核酸等物质的浓度变化会导致其吸收光谱的强度变化。于是采用紫外可见光分光光度法对肿瘤细胞进行测量,获取细胞周期各个阶段细胞内部组成成分的光谱表现形式。在获取的紫外可见光光谱数据中,可以找到光谱特征峰值随细胞周期的变化规律。实验结果说明紫外可见光光谱方法能够检测到肿瘤细胞细胞周期变化过程中的细胞内特定分子的变化,为细胞周期的研究提供了新思路。

The optical detection method which reflects the changes in the tumor cells growth process by researching on their spectra is obtained. According to the Beer-Lambert law,the changes of concentration of molecules such as aromatic amino acid and porphyrin in HeLa cells during the growth process of cell cycle will result in absorption spectra differences. Measuring the ultraviolet and visible absorption spectrum of the tumor cells can obtain the changes of spectrum during the different cell cycle growth phases. The relationship between cell cycle of tumor cells and characteristic peak of spectral curve can be acquired from the ultraviolet and visible absorption spectrum. The changes of intracellular specified molecules during the cell cycle of tumor cells can be detected by UV-visible spectral method. It provides new ideas for cell cycle studies.