为了研究健康中青年女性尿液荧光光谱的差异,采用SP-2558多功能光谱测量系统对200nm~390nm紫外光激发的20岁~55岁女性尿液进行测量,并运用多种软件对光谱数据进行多峰拟合处理和理论分析,得到了健康女性尿液荧光光谱的特性和产生机理。结果表明,紫外光激发女性晨尿产生的较强荧光光谱,主要是由尿液中的肾脏代谢的中间物质氧化型烟酰胺腺嘌呤核苷酸(nicotinamide ade-nine nucleotide,NAD+)、核黄素、粪卟啉3种物质产生,其对应的中心波长分别为458nm,520nm,612nm和674nm,后两者均是由粪卟啉产生的;随着年龄的增大,肾脏代谢功能下降,导致其尿样中肾脏代谢的中间物质NAD+的含量增加,从而使458nm谱峰强度明显增强。

In order to study the difference of urine fluorescence spectroscopy from healthy female,excited by 200nm~390nm ultraviolet light,the urine specimens of healthy women aging from 20~55 were measured with SP-2558 multifunctional spectrometer system.According to the multi-peak fitting on the line shape of spectrum,the characteristics and mechanism for urine spectrum were found.The results show that the strong urine fluorescence is caused by three inclusions:nicotinamide ade-nine nucleotide (NAD+) which is the metabolic intermediate of kidney,riboflavin and coproporphyrin.Their corresponding peaks are at 458nm,520nm,612nm and 674nm,and the latter two are generated by coproporphyrin.The difference between samples of youth and elderly is the peak intensity at 458nm,for the elderly women’s contends of NAD+ in the urine is higher than that of the young due to the degeneration of kidney metabolic function.