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女性尿液荧光光谱学特性及机理分析

Characteristics and mechanism for fluorescence spectroscopy of female urine

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摘要

为了研究健康中青年女性尿液荧光光谱的差异,采用SP-2558多功能光谱测量系统对200nm~390nm紫外光激发的20岁~55岁女性尿液进行测量,并运用多种软件对光谱数据进行多峰拟合处理和理论分析,得到了健康女性尿液荧光光谱的特性和产生机理。结果表明,紫外光激发女性晨尿产生的较强荧光光谱,主要是由尿液中的肾脏代谢的中间物质氧化型烟酰胺腺嘌呤核苷酸(nicotinamide ade-nine nucleotide,NAD+)、核黄素、粪卟啉3种物质产生,其对应的中心波长分别为458nm,520nm,612nm和674nm,后两者均是由粪卟啉产生的;随着年龄的增大,肾脏代谢功能下降,导致其尿样中肾脏代谢的中间物质NAD+的含量增加,从而使458nm谱峰强度明显增强。

Abstract

In order to study the difference of urine fluorescence spectroscopy from healthy female,excited by 200nm~390nm ultraviolet light,the urine specimens of healthy women aging from 20~55 were measured with SP-2558 multifunctional spectrometer system.According to the multi-peak fitting on the line shape of spectrum,the characteristics and mechanism for urine spectrum were found.The results show that the strong urine fluorescence is caused by three inclusions:nicotinamide ade-nine nucleotide (NAD+) which is the metabolic intermediate of kidney,riboflavin and coproporphyrin.Their corresponding peaks are at 458nm,520nm,612nm and 674nm,and the latter two are generated by coproporphyrin.The difference between samples of youth and elderly is the peak intensity at 458nm,for the elderly women’s contends of NAD+ in the urine is higher than that of the young due to the degeneration of kidney metabolic function.

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中图分类号:O433

所属栏目:光生物学与医学光子学

收稿日期:2008-09-22

修改稿日期:2008-11-04

网络出版日期:0001-01-01

作者单位    点击查看

陆俊:江南大学 理学院,无锡 214122
高淑梅:江南大学 理学院,无锡 214122
熊婕:江南大学 理学院,无锡 214122
杨幼益:无锡市妇幼保健医院,无锡 214122
陈国庆:江南大学 理学院,无锡 214122

联系人作者:陆俊(gaosm@jiangnan.edu.cn)

备注:陆俊|现主要从事激光光谱在医学上的应用研究|(1985-),女,硕士研究生。

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引用该论文

LU Jun,GAO Shu-mei,XIONG Jie,YANG You-yi,CHEN Guo-qing. Characteristics and mechanism for fluorescence spectroscopy of female urine[J]. Laser Technology, 2010, 34(1): 45-47

陆俊,高淑梅,熊婕,杨幼益,陈国庆. 女性尿液荧光光谱学特性及机理分析[J]. 激光技术, 2010, 34(1): 45-47

被引情况

【1】葛立新,高淑梅,吕思斌. 基于紫外光诱导血浆的三维同步荧光光谱共振能量转移及能量再吸收的分析. 光子学报, 2011, 40(10): 1500-1504

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