光谱学与光谱分析, 2009, 29 (7): 1826, 网络出版: 2010-05-26  

近红外技术在真菌生物量和真菌毒素检测中的研究与应用

Applications of Near Infrared Reflectance Spectroscopy in Detecting Chitin, Ergosterol and Mycotoxins
作者单位
1 江西农业大学农学院草业科学系, 江西 南昌330045
2 中国农业大学草地研究所, 北京100094
摘要
几丁质、 麦角甾醇和真菌毒素可以反应真菌侵染程度和危害性。 对几丁质、 麦角甾醇和真菌毒素进行监测, 可有效控制霉变的发生。 传统检测方法耗时、 繁琐, 而且不能及时有效地反映检测结果。 近红外光谱技术以其简便、 快捷等优越性广泛应用于不同领域, 在品质检测中有巨大的应用潜力和前景。 文章在介绍近红外技术(NIRS)的发展状况、 特点及其在品质分析上的应用的基础上, 着重阐述了近红外技术(NIRS)在真菌生物量(几丁质、 麦角甾醇)和真菌毒素检测中的研究和进展, 并对其应用前景进行了展望。 随着不同模型的定标方程的建立和模型数据库的不断完善, NIRS将会在真菌研究领域发挥越来越大的作用。
Abstract
The invasion extent and harmfulness of fungi can be determined by chitin, ergosterol and mycotoxins. It is important to monitor chitin, ergosterol and mycotoxins changes to prevent contamination of forage and feed products, and effectively control the sustainable development of the mildew. Predication of these chemical materials was often completed by laboratory analysis, which was time-consuming and cumbersome and could not reflect the results in time in the past. Near infrared reflectance spectroscopy (NIRS) is a rapid, convenient, highly efficient, nondestructive and low-cost analytical technique, which has been widely used in various fields such as food field and feed field for quantitative and qualitative analysis. It has a great potentiality of application in quality analysis. In this paper, the principle and the characteristic of NIRS and its applications in food, forage, feed and other agriculture products quality analysis were introduced. Its applications in fungal biomass (chitin, ergosterol) and mycotoxins were mainly reviewed. NIRS was used to quantify chitin, ergosterol and mycotoxins. Calibration equations and validation equations for these materials were developed. It is also expected that NIRS will play a more and more important role in the field of fungi with the establishment of calibration equation and improvement of model database.

易永艳, 李德荣, 张蕴薇, 杨富裕. 近红外技术在真菌生物量和真菌毒素检测中的研究与应用[J]. 光谱学与光谱分析, 2009, 29(7): 1826. YI Yong-yan, LI De-rong, ZHANG Yun-wei, YANG Fu-yu. Applications of Near Infrared Reflectance Spectroscopy in Detecting Chitin, Ergosterol and Mycotoxins[J]. Spectroscopy and Spectral Analysis, 2009, 29(7): 1826.

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