光谱学与光谱分析, 2009, 29 (7): 1851, 网络出版: 2010-05-26
不同产地葛根红外光谱的三级鉴定
Identification of Three-Step IR Spectra of Radix Puerariae from Different Habitats
葛根 红外光谱 二阶导数谱 二维相关红外光谱 鉴别 Radix Puerariae IR spectra Second derivative spectra 2D-IR correlation spectra Identification
摘要
运用红外光谱法和二维相关光谱技术对天津八仙山、 湖南壶瓶山、 重庆金佛山三种产地的葛根样本进行了分析鉴定。 分析结果表明: 不同产地葛根的红外光谱(一级鉴定)和二阶导数谱(二级鉴定)具有一定的相似度, 与淀粉和葛根素的谱图比对, 三个样本都富含淀粉和一定量的葛根素, 不同的是天津葛根在1 048 cm-1波数处的最强峰与淀粉在986 cm-1处的最强峰相差了62个波数, 说明淀粉的含量较低, 而在891, 835和797 cm-1的特征峰与葛根素的指纹特征更一致, 可见葛根素的含量要高一些。 在二维相关谱图(三级鉴定)上, 三种产地的葛根形成的峰簇的位置和数量不同, 可以进行产地的鉴别。 结果表明, 对于不同产地葛根的分析, 红外光谱法的三级鉴定是一种有效和快速的检测方法。
Abstract
The IR spectra method and 2D-IR correlation spectra method were used for the identification of radix puerariae from Baxian mountain in Tianjin, Huping mountain in Hunan and Jinfo mountain in Chongqing. The results show that the IR spectra (the first identification) and the second derivative spectra (the sccond identification) of kudzu roots from different habitats have some similarity. Compared with the spectra of amylum and puerarin, three samples are all rich in amylum, and have some puerarin. The primary difference is the 62 wave numbers between the strongest peak of Tianjin kudzu at 1 048 cm-1 and amylum at 986 cm-1, showing that the content of amylum is lower. Radix puerariae from Tianjin has characteristic peaks consistent with those of puerarin at 891, 835 and 797 cm-1, which means that radix puerariae from Tianjin has more puerarin. In the 2D-IR correlation spectra (the third identification), the three kinds of radix puerariae have the difference in position and the intensity of the correlation peak cluster. Therefore, the three-step IR macro-fingerprint provides a rapid and effective method for the identification of radix puerariae from the different habitats.
韩明霞, 周群, 李全宏, 孙素琴. 不同产地葛根红外光谱的三级鉴定[J]. 光谱学与光谱分析, 2009, 29(7): 1851. HAN Ming-xia, ZHOU Qun, LI Quan-hong, SUN Su-qin. Identification of Three-Step IR Spectra of Radix Puerariae from Different Habitats[J]. Spectroscopy and Spectral Analysis, 2009, 29(7): 1851.