光谱学与光谱分析, 2009, 29 (8): 2079, 网络出版: 2010-05-26  

近红外光谱测定猪眼肌肌内脂肪中脂肪酸含量

Detection of Fatty Acid Composition in Intramuscular Fat of Packed Pork Loin by Near Infrared Spectroscopy
作者单位
1 西北农林科技大学机电学院, 陕西 杨凌712100
2 日本全农中央畜产研究所, 茨城县筑波, 3004204
3 日本筑波大学, 茨城县筑波, 305-8702
摘要
为了快速无损测定猪眼肌肌内脂肪中脂肪酸含量,提出了近红外光谱技术检测的新方法。 选用气相色谱法作为猪眼肌肌内脂肪中脂肪酸测定的标定方法,采用漫反射光纤探头采集由低密度聚乙烯塑料袋包装的猪眼肌样品的光谱, 来测定眼肌内脂肪中脂肪酸的含量。 利用光谱专用分析软件Unscrambler9.6, 对采集的光谱用平滑, 二阶微分法进行预处理, 用偏最小二乘法(PLS)建立其定量检测模型。 用于本实验的样本总数为82, 将异常点除去后, 将样品分为校正集和检验集。 用校正集的样品来建立定标方程, 再用它来预测检验集样品的值。 结果表明, 脂肪酸C14∶0, C15∶1, C16∶0, C16∶1, C18∶0, C18∶1, C18∶2, C18∶3, C20∶1, C20∶4, SFA, MUFA和PUFA校正模型的相关系数分别为0.57, 0.76, 0.71, 0.77, 0.62, 0.81, 0.86, 0.91, 0.85, 0.91, 0.67, 0.81和0.95。 可见, 用该方法测定肌内脂肪中脂肪酸有较高的测定精度, 因此, 应用近红外光谱对猪眼肌肌内脂肪中脂肪酸含量的快速无损检测具有可行性。
Abstract
Near infrared spectroscopy as a new method was proposed for rapid and non-destructive measurement of fatty acid composition in intramuscular fat of packed pork loin. Gas chromatography was used as a reference method for the spectral analysis of fatty acid composition. The fiber optic in interactance mode was adopted to measure the spectra of packed pork loins by low density polythene. The original spectra were pretreated by smoothing and 2nd derivative, and then PLS calibration model was builtby using software of Unscrambler 9.6. A total of eighty two samples were used in the experiment. The samples were divided into calibration set and validation set after removing the outliers. The calibration set was used to set up calibration model and then the model was adopted to predict the samples of validation set. The results show that the correlation coefficient for C14∶0, C15∶1, C16∶0, C16∶1, C18∶0, C18∶1, C18∶2, C18∶3, C20∶1, C20∶4, SFA, MUFA, PUFA is 0.57, 0.76, 0.71, 0.77, 0.62, 0.81, 0.86, 0.91, 0.85, 0.91, 0.67, 0.81 and 0.95, respectively. It means that evaluating fatty acid using near infrared spectroscopy in interactance mode has higher precision. Near infrared spectroscopy technique is a feasible and rapid method for nondestructive detection of fatty acid composition in intramuscular fat of packed pork loin.

胡耀华, 郭康权, 野口刚, 佐竹隆显. 近红外光谱测定猪眼肌肌内脂肪中脂肪酸含量[J]. 光谱学与光谱分析, 2009, 29(8): 2079. HU Yao-hua, GUO Kang-quan, NOGUCHI Gou, SATAKE Takaaki. Detection of Fatty Acid Composition in Intramuscular Fat of Packed Pork Loin by Near Infrared Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2009, 29(8): 2079.

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