光谱学与光谱分析, 2009, 29 (9): 2351, 网络出版: 2010-05-26  

基于太赫兹光谱的氨基酸检测

Detection of Amino Acids Based on Terahertz Spectroscopy
作者单位
1 广西工学院生物与化学工程系, 广西 柳州545006
2 中国科学院上海应用物理研究所, 上海201800
3 柳州市圣诺化工有限责任公司, 广西 柳州545006
摘要
太赫兹是指频率从0.1到2.0 THz之间的远红外波。 与傅里叶红外相比, 太赫兹时域光谱能量低, 信躁比高, 并且无辐射损伤。 氨基酸分子的低频振动模式(扭转, 集体振动模式和氢键)处在 THz波段。 氨基酸是一类重要的生物分子, 是组成蛋白质最基本的物质。 氨基酸分子以分子间氢键相互连接构成晶体。 氨基酸在 THz波段比在红外波段体现更多独特吸收特征。 到目前为止, 已经获得了20种氨基酸分子的太赫兹吸收谱, 包括利用太赫兹技术对部分氨基酸的定量分析。 氨基酸的太赫兹光谱研究, 有利于深层次理解蛋白质/ DNA的低频振动模式及相关生物反应和活性。 文章综述了20种氨基酸分子的太赫兹吸收光谱并建立了吸收光谱数据库。 总结了太赫兹技术在氨基酸应用方面存在的问题, 并对未来发展方向进行展望。
Abstract
Terahertz (THz) is the frequency region ranging from 0.1 to 2.0 THz, which lies in the far-infrared region. Compared to Fourier transform infrared spectra (FTIR), terahertz time-domain spectra (THz-TDS) has low energy, high signal-to-noise ratio (SNR) and is non-ionizing radiation. Low-frequency vibrational modes of some amino acids, such as torsional and collective vibrational modes and hydrogen-bond modes, exist in the THz region. Amino acids are important organic compounds and are the fundamental components of proteins. Amino acids can exist with a highly ordered crystal structure linked by hydrogen intermolecular bonds in the solid phase. The absorption spectra of amino acids in the THz region show marked differences while mid-infrared absorption spectra usually show very little difference. Up to now, absorption spectra of twenty kinds of amino acids have been studied by many researchers using THz technique; the quantitative analysis of amino acids by THZ-TDS is also included. Investigation of THz spectra of amino acids are of fundamental interests, and will lead to further understanding of low-frequency vibrations of protein/DNA and relevant biological reactions and activities. In the present paper, the latest progress in absorption spectra of amino acids determined by THz spectroscopy is reviewed and a database is built. Some brief remarks on future developments in and prospects for THz application in amino acids are also provided.

唐忠锋, 林海涛, 陈晓伟, 张增芳. 基于太赫兹光谱的氨基酸检测[J]. 光谱学与光谱分析, 2009, 29(9): 2351. TANG Zhong-feng, LIN Hai-tao, CHEN Xiao-wei, ZHANG Zeng-fang. Detection of Amino Acids Based on Terahertz Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2009, 29(9): 2351.

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