不具有荧光的探针双氢罗丹明6G被氧化后可以生成具有荧光特性的罗丹明6G。反应中，一个双氢罗丹明分子能够与2个活性氧发生作用。借助衍生荧光检测技术，可以将该探针用于香烟主流烟雾活性氧含量的检测实验。利用探针在该实验条件下检测市场上4种品牌香烟的主流烟雾中活性氧的含量，含量分别为：(59.93±5.32)nmol，(55.98±6.17)nmol，(54.78±7.82)nmol，(40.87±6.43)nmol。实验结果表明，香烟主流烟雾中活性氧的含量与烟草品种及制作工艺存在密切的联系。借助荧光特性进行主流烟雾活性氧含量近实时检测的方法具有灵敏度高与检测速度快的特点。

Non-fluorescence probe dihydrorhodamine 6G (dR6G) can be oxidized to fluorescent rhodamine 6G. In the process, each molecule reacts with two active oxygen molecules. The probe can be used to detect ROS in mainstream cigarette smoke by using derivative fluorescence method. We detected the contents of ROS in mainstream smoke from the 4 types of cigarettes purchased in market. The ROS content is (59.93±5.32)nmol,(55.98±6.17)nmol,(54.78±7.82)nmol, (40.87±6.43)nmol respectively. The results show that the amount of ROS is dependent on the types of tobacco and cigarette production technology. This real-time ROS detection method in mainstream smoke by fluorescence characteristic is fast and sensitive.