光学学报, 2010, 30 (7): 2136, 网络出版: 2010-07-13
获取生物分子完整拉曼谱的时间分辨相干反斯托克斯拉曼散射方法的理论研究
Theoretical Analysis of Time-Resolved Coherent Anti-Stokes Raman Scattering Method for Obtaining the Whole Raman Spectrum of Biomolecules
光谱学 相干反斯托克斯拉曼散射(CARS) 非标记分子识别 非共振背景噪声 时间分辨方法 宽谱激光光源 spectroscopy coherent anti-Stokes Raman scattering(CARS) non-labeling molecular identification nonresonant background noise(NRB) time-resolved method wide-spectrum laser source
摘要
利用半经典理论分析了三束中心频率不同的窄线宽激光光场在样品中的相互耦合过程,得到一组描述相干反斯托克斯拉曼散射(CARS)过程的耦合波方程。结合时间分辨方法的原理,得到了描述使用窄线宽激光脉冲的时间分辨CARS(T-CARS)方法的耦合波方程。采用数值模拟方法研究了T-CARS方法中CARS信号和非共振背景噪声与入射光场之间的关系以及分子振动的弛豫过程。说明分子振动的退相时间可以作为区分成份和结构相近的分子以及监测样品所处微环境变化的依据。基于使用窄线宽激光光源的T-CARS方法的理论分析说明了使用宽谱激光具有同时获取生物分子完整拉曼光谱的能力。
Abstract
The mixing process of three narrow line-width laser beams with different wavelengths in the sample is analyzed with semi-classical theory and a set of coupling-wave equations for describing the generation process of coherent anti-Stokes Raman Scattering (CARS) signals is derived. Based on the derived CARS coupling-wave equations and the principle of the time-resolved method,a set of coupling-wave equations for describing the time-resolved CARS (T-CARS) method is obtained. The variation of CARS signals and non-resonant background noise with incident laser beams in the T-CARS method and the decaying process of molecular vibrations have been investigated by numerical simulation. The decaying time of molecular vibrations can be used to distinguish different molecules with similar components and structure,and monitor the change of microenvironment. Theoretical study of T-CARS method using narrow line-width laser source shows that whole Raman spectra of biomolecules can be obtained by using wide-spectrum laser source.
尹君, 于凌尧, 屈军乐, 牛憨笨, 林子扬. 获取生物分子完整拉曼谱的时间分辨相干反斯托克斯拉曼散射方法的理论研究[J]. 光学学报, 2010, 30(7): 2136. Yin Jun, Yu Lingyao, Qu Junle, Niu Hanben, Lin Ziyang. Theoretical Analysis of Time-Resolved Coherent Anti-Stokes Raman Scattering Method for Obtaining the Whole Raman Spectrum of Biomolecules[J]. Acta Optica Sinica, 2010, 30(7): 2136.