中国光学, 2009, 2 (5): 426, 网络出版: 2010-09-07  

白蛋白酸致变性的动态激光散斑研究

Investigation of acid induced denaturation of albumin by dynamic laser speckle method
作者单位
河南科技大学 理学院,河南 洛阳 471003
摘要
利用动态激光散斑法研究了白蛋白的酸致变性过程。基于动态散斑理论模拟生成了动态散斑序列图;利用模拟和实验散斑图生成的时间序列散斑图及其灰度共生矩阵对白蛋白的酸致变性过程进行了分析;最后用灰度共生矩阵的惯性矩和不同阶段的4幅散斑图的平均对比度图分析了不同阶段白蛋白胶体溶液中微粒数量和尺寸的变化。理论模拟和实验研究显示,在白蛋白酸致变性过程中,胶体溶液中的蛋白质微粒的运动由剧烈逐渐变得缓慢;同时,微粒数量减少而微粒尺寸增加。研究结果表明,动态激光散斑法是一种实时、快速、有效地研究白蛋白变性过程的新型检测手段。
Abstract
The process of the acid-induced denaturation of albumin was studied by using a dynamic laser speckle method in this paper. The dynamic speckle pattern sequences were generated based on the dynamic speckle theory, then the acid induced denaturation process of the albumin was analyzed with the Time History Speckle Patterns(THSP) and its Gray Level Co-occurrence Matrix(GLCM) obtained from simulation and experiments. Furthermore, this denaturation-induced process was investigated by using the inertia moment of the GLCM and the mean contrast methods. The theoretical simulation and the experiment results inducate that the protein particle movement changes from high activity to low activity gradually in the colloid solution during the denaturation process, meanwhile, the quantity of the protein particles decreases while its size increases. These results show that the dynamic laser speckle method is a real-time, quick and effective method to study the acid-induced denaturation of the albumin.

李新忠, 甄志强, 李立本, 陈庆东. 白蛋白酸致变性的动态激光散斑研究[J]. 中国光学, 2009, 2(5): 426. LI Xin-zhong, ZHEN Zhi-qiang, LI Li-ben, CHEN Qing-dong. Investigation of acid induced denaturation of albumin by dynamic laser speckle method[J]. Chinese Optics, 2009, 2(5): 426.

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