考虑传统激光显微操纵器的缺点，采用反射式结构设计了新的激光显微操纵器，以提高激光显微操纵器输出光斑的质量。用He-Ne激光作为指示光束，CO2激光作为工作光束，对两种光斑的重合性、光斑大小进行了优化设计；利用ZEMAX软件中多重结构的功能使系统设计满足200～400 mm工作距离可调的要求。对设计的系统进行了实验，给出了光学系统图、能量图及点列图表。实验结果表明，在规定的工作距离内，系统的光斑重合性，光束均匀性、稳定性及可靠性等方面均有了很大提高。该激光显微操纵器与手术显微镜结合，有术野清晰，操作安全的特点，可满足医疗的长期使用要求。

In consideration of the shortcomings of traditional laser micro-manipulators, a laser micro-manipulator with the reflection structure was designed to improve the quality and reliability of spots. By taking a He-Ne laser as its indication light beam and a CO2 laser as its working light beam, the repeatability and sizes of the two spots were optimized. Furthermore, using the multi-configuration of a ZEMAX, the adjustable performance of working distance was realized from 200 mm to 400 mm. By carring out the corresponding experiment, then the optical system chart, diffraction encircled energy diagram and the spot diagram were given. The experimental results show that the repeatability of spots and the uniformity, stability and reliability of beams are improved a lot. With the characteristics of its clear operational visual field and treated safety, the laser micromanipulator can be combined with the operation microscopy to meet the long-term use need of the treatment.