研究CdTe量子点(供体)和罗丹明B(受体)之间荧光共振能量转移的最佳条件， 建立了荧光共振能量转移猝灭法测定金银花中微量铜的新方法。 采用十六烷基三甲基溴化铵， 在pH 6.00的Tris-HCl缓冲液中， Cu2+能对能量转移体系中罗丹明B荧光峰强猝灭从而测定铜的含量。 Cu2+浓度在1.3×10-4～3.1×10-2 μg·mL-1范围内与罗丹明B的荧光强度变化ΔF呈现良好线性关系(r=0.996)， 检测限为4.6×10-5 μg·mL-1， RSD为3.5%， 平均回收率为103.4%(n=5)。 该方法适用于金银花中微量铜的测定。

The present work is to find the optimum condition for fluorescence resonance energy transfer between CdTe QDs (donor) and rhodamine B (acceptor) and form a new method to detect copper in Flos Lonicerae Japonicae. In tris-HCl buffer solution at pH 6.00, by employing cetyltrimethylammonium bromide, Cu2+ can quench the fluorescence intensity of BRB and detect the concentration of Cu2+. Results show that a linear relationship could be established between the quenched fluorescence intensity of RhB and the concentration of Cu2+ in the range of 1.3×10-4～3.1×10-2 μg·mL-1, the limit of detection was 4.6×10-5 μg·mL-1 (r=0.996), RSD and the average recovery was 3.5% and 103.4% (n=5), respectively. It is concluded that the developed method was proved to be feasible to detect copper in Flos Lonicerae Japonicae.