光学学报, 2011, 31 (s1): s100118, 网络出版: 2011-06-23
水溶性苄叉环戊酮染料的双光子光动力性能研究
Study on Water-Soluble Benzylidene Cyclopentanone Dyes for Two-Photon Induced Photodynamic Therapy
医用光学 光动力治疗 双光子激发 苄叉环戊酮 活性氧物种 medical optics photodynamic therapy two-photon excitation benzylidene cyclopentanone reactive oxygen species
摘要
通过在苄叉环戊酮分子骨架外围引入不同数目羧酸钠基团,合成了4种水溶性的苄叉环戊酮染料,系统研究了它们的光物理和光化学性质。结果表明4种染料在修饰后水溶性都大幅提升。它们在近红外波段都具有较大的双光子吸收截面,而且在可见光照射下均能快速产生单线态氧和超氧负离子两种活性氧物种。单光子光动力实验结果表明4种新染料的暗毒性都较低,但只有Y1展现出强的光动力活性,说明保持适度的脂溶性对光敏药物非常重要。进一步的细胞实验显示,染料Y1对人直肠癌1116细胞产生了明显的双光子损伤效应。这表明Y1在双光子光动力治疗上有很大的应用潜力。
Abstract
Four water-soluble benzylidene cyclopentanone dyes are synthesized for photodynamic therapy (PDT) by introducing different numbers of carboxylate groups on their periphery. Their photophysical and photochemical properties are investigated systematically. The results show that the water solubility of these dyes is significantly enhanced after modification. All dyes exhibit large two-photo absorption (TPA) cross-section in near infrared (IR) range, which can generate both superoxide anion radical and singlet oxygen species under visible illumination. One-photon PDT experiments indicate that four novel dyes displaye low dark cytotoxicity. However, only the dye Y1 presents excellent photodynamic activity, which highlights the role of moderate lipophilicity for PDT dyes. Further cellular experiments reveals that Y1 had obvious two-photon induced cytotoxic effect on human rectal cancer 1116 cells. The result indicates that Y1 is a potential photosensitizer for two-photon induced PDT.
杨威, 邹千里, 周扬, 顾瑛, 赵榆霞, 吴飞鹏. 水溶性苄叉环戊酮染料的双光子光动力性能研究[J]. 光学学报, 2011, 31(s1): s100118. Yang Wei, Zou Qianli, Zhou Yang, Gu Ying, Zhao Yuxia, Wu Feipeng. Study on Water-Soluble Benzylidene Cyclopentanone Dyes for Two-Photon Induced Photodynamic Therapy[J]. Acta Optica Sinica, 2011, 31(s1): s100118.