可见光波段激光“多色多光子光谱技术”应用到生物样品方面可替代紫外光, 因为紫外光容易将生物分子打成碎片而破坏生物分子。 可见光波段的激光多色多光子有选择地多步激发同样可达到紫外光所激发的能级位置, 甚至可达到更高的激发能位, 并不破坏生物样品, 分辨率﹑灵敏度﹑选择性和探测目标的准确性更高。 用一束二倍频Nd∶YAG激光(重复频率10次·s-1)同步泵浦三台脉冲可调谐染料激光器、 含微通道板组件的飞行时间质谱仪、 纳秒示波器和Boxcar等测量了UI的多色三光子共振光电离谱和高时间分辨激光光谱； 文章多项创新点在于: 首次用激光诱导量子布居的图型法, 给出了在三色三光子共振光电离实验中单色和双色三光子共振光电离谱峰产生的原因； 解决了如何避免产生单色和双色峰的问题, 也就是解决了如何避免产生“假峰”的问题, 使多色三光子共振光电离纯度显著提高； 在此基础上, 不仅将相距很近、 能级位置仅差0.642 cm-1、 很难分开的铀同位素A和B两个谱峰很好地分开了, 而且分别测得了这两个能级的激发态寿命值等。 此技术不仅限于铀光谱研究, 更重要的是提供了它的通用性新信息。 这项基础研究新方法和新技术可扩展到生物和医药样品激光检测和分析研究领域。

The multicolour three-photon resonant photoionization spectra and high-time-resolved laser spectrum of UI were measured with a setup composed of a Nd∶YAG-laser(532 nm, operated at 10 Hz)-pumped pulsed tunable dye laser system, a time-of-flight mass spectrometer, including micro-channel plate components, ns-oscilloscope, boxcar integrator, and so on. Creative inventions of this paper are for the first time by laser-induced quantum population of the graphic method, the causes for single-colour and two-colour three-photon resonant photoionization spectra peak were given in the three-colour three-photon resonant photoionization experiment； The question how to avoid producing single-colour and two-colour three-photon resonant photoionization spectra peak was solved, That is, how to solve the problem to avoid “false peaks”, so that multicolour three-photon resonant photoionization purity was raised remarkably; On this basis, not only in close proximity to energy level position with just a difference 0.642 cm-1, the isotopes A and B of uranium, which are difficult to distinguish, were well resolved, but the two excited state lifetime values were obtained respectively. This technology is not limited to uranium spectrum, but more importantly, it’s versatile. The new methods and technologies of basic research can be expanded to samples of biological and medical research fields with laser detecting and analysis.