提供了一种能实时快捷地对光镊系统中四象限探测器位移探测信号进行校准的方法。实验研究表明了该方法的可靠、精确，其自身的不稳定性小于1%。利用该方法研究了校准系数随小球深度的变化情况，得出校准系数随小球深度的升高而减小。该方法可以用于光镊系统稳定性的监测。

A method for real-time, quick calibration of four-quadrant photodetector (QD) signals in optical tweezers system is provided. The experimental results indicate that the method is feasible and precise. Its instability is less than 1%. In addition, the effect of the bead depth on calibration factor is investigated experimentally, and it is found that the calibration factor decreases when the bead depth increases. The method can be used to monitor the stability of the optical tweezers system.