发光学报, 2011, 32 (10): 993, 网络出版: 2011-10-21   

聚乙烯吡咯烷酮修饰的NaYF4∶Yb3+/Er3+上转换发光纳米粒子的制备及其生物兼容性

Synthesis of Water Soluble Upconversion NaYF4∶Yb3+/Er3+ Nanoparticals Using Polyvinylpyrrolidone as Surfactant and Their Biological Compatibility
作者单位
1 吉林大学 第一医院泌尿外科, 吉林 长春 130022
2 吉林大学电子科学与工程学院 集成光电子国家重点联合实验室, 吉林 长春 130021
摘要
以聚乙烯吡咯烷酮(PVP)为表面活性剂, 利用溶剂热法合成了NaYF4∶20%Yb3+, 3%Er3+(摩尔分数)的上转换发光纳米粒子。利用扫描电子显微镜对粒子的形貌进行了表征, 结果表明纳米颗粒的尺寸在30~40 nm, 分布比较均匀。在980 nm红外光的激发下, 样品能够发出肉眼可见的明亮的黄色上转换荧光。样品可以较好地分散在水溶液中形成透明澄清的溶液。利用MTT实验测量了不同给药浓度下NaYF4纳米粒子是否对HeLa细胞具有生物毒性。结果表明: PVP修饰的NaYF4∶Yb3+/Er3+上转换发光纳米粒子具有较好的生物兼容性, 对HeLa细胞无生物毒性, 在生物荧光标记领域具有潜在的应用价值。
Abstract
Water soluble NaYF4∶20%Yb3+,3%Er3+ (mole fraction) upconversion nanoparticles (UCNPs) were synthesized by facile solvothermal method using polyvinylpyrrolidone (PVP) as the surfactant. The size and morphology of the UCNPs were confirmed by scanning electron microscopy (SEM). The SEM images showed almost all the UCNPs were nanospheres and the size was about 40 nm. Under the 980 nm excitation, the UCNPs exhibit bright yellow light. The UCNPs could be well dispersed in water to form a clearly transparent solution. MTT assay was performed to analyze the biotoxicity of the UCNPs. The results showed the NaYF4∶Yb3+/Er3+ UCNPs modified by PVP had fairly biological compatibility and no biotoxicity for HeLa cell line, even the concentration of UCNPs reached 200 mg/mL. These results showed that the UCNPs had potential applications in biological field as luminescence labeling probes.

芦志华, 陈欢, 何春凤, 揣晓红, 秦冠仕, 赵丹. 聚乙烯吡咯烷酮修饰的NaYF4∶Yb3+/Er3+上转换发光纳米粒子的制备及其生物兼容性[J]. 发光学报, 2011, 32(10): 993. LU Zhi-hua, CHEN Huan, HE Chun-feng, CHUAI Xiao-hong, QIN Guan-shi, ZHAO Dan. Synthesis of Water Soluble Upconversion NaYF4∶Yb3+/Er3+ Nanoparticals Using Polyvinylpyrrolidone as Surfactant and Their Biological Compatibility[J]. Chinese Journal of Luminescence, 2011, 32(10): 993.

本文已被 3 篇论文引用
被引统计数据来源于中国光学期刊网
引用该论文: TXT   |   EndNote

相关论文

加载中...

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!