提出一种利用高斯函数来表征基于扫描相机的时域荧光测量系统脉冲响应的方法，建立了数学模型，用以描述系统所采集到的时域荧光衰减曲线。用高斯函数对所建立的时域荧光寿命测量系统的脉冲响应函数进行拟合处理，拟合优度达到0.995以上；利用所建立的数学模型对标准荧光染料玫瑰红的测量数据进行拟合处理，拟合优度均值达到0.996以上，荧光寿命标准差仅有1.7 ps。实验表明，利用所建立的数学模型处理基于扫描相机的时域荧光寿命测量数据，具有良好的稳定性和准确性。这种方法提高了系统测量的精度，简化了解卷积运算，只要用多个高斯函数描述脉冲响应函数就可以用于其他时域荧光寿命分析及荧光寿命成像技术中。

A method which describes the impulse response function of the fluorescence lifetime measurement system that based on a streak camera using Gaussian function is presented, and a mathematical model which is used to fit the fluorescence decay curves in time-domain is established. The data of the impulse response function of the time-domain fluorescence lifetime measurement system built using the Gaussian function are fit. The goodness of the fitting is greater than 0.995. The fluorescence lifetime of the standard fluorescent dye Rose Bengal is measured and the data using the mathematical model are fit. The average goodness of fitting is greater than 0.996 and the standard deviation of lifetime is only 1.7 ps. Experimental results show that the mathematical model is reliable and accurate in processing the time-domain signal obtained by fluorescence lifetime measurement system that is based on a streak camera. The method presented simplifies the deconvolution calculation, improves the precision of lifetime measurement and can be used to process time-domain signals obtained by other systems.