双光子荧光显微成像技术具有较低的光漂白与光损伤、较佳的成像对比度、较深的穿透深度等优点。采用结扎大鼠冠状动脉左前降支的方法建立急性心肌缺血模型，通过双光子荧光显微成像技术获得不同结扎时间下大鼠左心室前壁心肌组织的双光子荧光图像。从中分析了不同结扎时间下心肌组织的形态学变化，实现对心肌组织损伤范围的确定；并对双光子荧光图像进行快速傅里叶变换(FFT)，使用方向指数这一参数对心肌缺血的程度进行初步的量化评估。结果表明,双光子荧光显微成像技术结合FFT分析技术,有望在心肌缺血性疾病的诊断和治疗中实时、快速、准确地判断心肌缺血的范围和评估其损伤程度。

Two-photon fluorescence imaging technology has several outstanding characteristics, including lower photon-bleaching and photo-damaging, high contrast and longer penetrating distance. An acute myocardial ischemia model of rat is established by the ligation of the left anterior descending branch. Then fluorescence images of the front wall of left ventricle at different ligation time are attained by two-photon fluorescence imaging. The morphologic change of myocardial ischemia tissue was obtained and a primary evaluation of the damage degree of myocardial tissue is obtained by fast Fourier transform (FFT) with an orientation index. The result indicates that the two-photon fluorescence imaging technology combined with FFT has the potential to be used for the estimation of myocardial ischemia tissue range and assess of the damage degree of myocardial tissue rapidly and accurately in the diagnosis and therapy in real time.