大气与环境光学学报, 2011, 6 (6): 443, 网络出版: 2011-12-01  

基于特征荧光光谱的铜绿微囊藻MC-LR的快速预测

Rapid Estimation on Microcystin-LR Concentration Released by Microcystic Aeruginosa Using Feature Fluorescence Spectroscopy
作者单位
1 安徽农业大学资源与环境学院, 安徽 合肥 230001
2 中国科学院安徽光学精密机械研究所 中国科学院环境光学与技术重点实验室, 安徽 合肥 230031
摘要
以自然水体为培养基对铜绿微囊藻(Microcystic aeruginosa)进行了为期95天的实验室培养。测量了铜绿微囊 藻不同生长阶段培养水体的三维荧光光谱和同步扫描荧光光谱,并且同步分析了藻类叶绿素a浓度和胞外微囊藻毒 素MC-LR浓度。结果表明:根据三维荧光光谱得到荧光信号A、信号X和信号C,分别代表类蛋白、海洋腐殖酸和类 富里酸。对各荧光信号强度与细胞外微囊藻毒素MC-LR浓度以叶绿素a浓度进行归一化,发现二者在藻类衰亡期具 有相同的变化趋势,且最大值出现的时间较为一致,两者表现出显著的线性相关性。因此,结合荧光光谱特征信 号和叶绿素a浓度可以实现对藻毒素MC-LR浓度的快速预测。就实验方法而言, Δλ=80 nm的同步扫描荧光光谱法 相对于三维荧光光谱法具有耗时少、测量简便等优点,可以完整反映荧光信号A、X和C的强度,更适合用于以藻 毒素快速预测为目的的水体荧光光谱在线分析。
Abstract
Microcystic aeruginosa was cultivated in laboratory condition with nature water for about 95 days. Three dimensional excitation-emission (3DEEM) fluorescence spectroscopy and synchronous scan fluorescence spectroscopy of the culture water at different growth stages were measured, simultaneously the chlorophyll-a and extracellular MC-LR concentration were analyzed. The results show that fluorescence signal A, signal X and signal C could be observed obviously from 3DEEM fluorescence spectroscopy and synchronous scan fluorescence spectroscopy, which represent protein-like substance, marine humic-like acid and fulvic-like substance respectively. All the three fluorescence signals intensity and MC-LR concentration were normalized by chlorophyll-a concentration. In the decline phase, the normalized fluorescence signals(A, X, C) and normalized MC-LR concentration show the same dynamic laws and good linear correlation. The maximum values appeared at the same time. Therefore, MC-LR concentration can be estimated by the feature fluorescence signal (A, X, C) and chlorophyll-a concentration. Comparing to 3DEEM fluorescence spectroscopy, synchronous scans fluorescence spectroscopy (Δλ=80 nm) is less time-consuming and can give out the entire feature fluorescence signal (A, X, C). It is suitable for real-time analysis of water fluorescent spectroscopy which can be used to rapidly estimate the MC-LR concentration.

段静波, 花日茂, 刘文清, 张玉钧, 王志刚, 肖雪, 王欢博. 基于特征荧光光谱的铜绿微囊藻MC-LR的快速预测[J]. 大气与环境光学学报, 2011, 6(6): 443. DUAN Jing-bo, HUA Ri-mao, LIU Wen-qin, ZHANG Yu-jun, WANG Zhi-gang, XIAO Xue, WANG Huan-bo. Rapid Estimation on Microcystin-LR Concentration Released by Microcystic Aeruginosa Using Feature Fluorescence Spectroscopy[J]. Journal of Atmospheric and Environmental Optics, 2011, 6(6): 443.

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