中国激光, 2020, 47 (2): 0207004, 网络出版: 2020-02-21
光学相干层析显微成像的技术与应用 下载: 2945次特邀综述
Optical Coherence Microscopy and Its Application
医用光学 光学相干层析显微成像 生物医学成像 共聚焦显微成像 超高分辨率 深层组织成像 medical optics optical coherence microscopy biomedical imaging confocal microscopy ultrahigh resolution deep tissue imaging
摘要
光学相干层析显微成像(OCM)技术是一种使用相干探测的光学显微成像技术。OCM不仅具有光学相干层析成像技术(OCT)高轴向分辨、高信噪比、无需标记的优势,而且能通过高倍物镜获得高横向分辨能力,能实现微米量级的空间分辨率。首先介绍OCM技术的基本原理和实现方案,然后详细阐述OCM技术的原理以及在国际上的研究进展。针对OCM技术中如何实现超高分辨成像、焦深限制成像深度等问题,对目前该研究领域一些先进的OCM技术进行总结。OCM技术在生物医学、材料检测等领域具有广泛的应用前景。
Abstract
Optical coherence microscopy (OCM) utilizes a coherent detection method for optical microscopy. Having advantages, such as high axial resolution, high signal-to-noise ratio, and label-free imaging in optical coherence tomography (OCT), the OCM can achieve a micron-scale spatial resolution by utilizing a high-power objective for obtaining high lateral resolution. Initially, the basic principle and implementation scheme of the OCM technology are introduced; subsequently, the principles and research progress of the OCM technology around the world are summarized. This study also examines some advanced OCM technologies considering the problems that how to realize ultra-high-resolution imaging and that the depth of focus limits the imaging depth. The OCM technology has broad application prospect in biomedicine, material detection, and other fields.
韩涛, 邱建榕, 王迪, 孟佳, 刘智毅, 丁志华. 光学相干层析显微成像的技术与应用[J]. 中国激光, 2020, 47(2): 0207004. Han Tao, Qiu Jianrong, Wang Di, Meng Jia, Liu Zhiyi, Ding Zhihua. Optical Coherence Microscopy and Its Application[J]. Chinese Journal of Lasers, 2020, 47(2): 0207004.