光谱学与光谱分析, 2012, 32 (5): 1266, 网络出版: 2012-06-14   

基于表面增强拉曼光谱技术的豆芽6-BA残留快速检测方法

Rapid Detection of 6-Benzylaminopurine Residues in Sprout Beans by Surface-Enhanced Raman Spectroscopy
作者单位
1 北京工业大学生命科学与生物工程学院, 北京100124
2 欧普图斯(苏州)光学纳米科技有限公司, 江苏 苏州215125
摘要
采用快速溶剂提取前处理技术与表面增强拉曼光谱(surface-enhanced Raman spectroscopy, SERS)检测技术建立豆芽中6-苄基腺嘌呤(6-benzylaminopurine, 6-BA)残留物的快速定量检测方法。 以自发豆芽为空白对照, 通过对梯度浓度的6-BA插标豆芽提取液进行SERS检测发现, 在1 002 cm-1处具有明显的6-BA特征拉曼峰, 且归一化处理后的特征峰峰强与6-BA浓度呈良好的线性关系, 高浓度与低浓度的线性范围分别为0.5~14 μg·mL-1与0.1~2 μg·mL-1, 最低定量检出限为0.02 mg·kg-1, 回收率为82.3%~95.1%, 相对标准偏差小于5%。 该方法重现性较好、 前处理简单、 全程检测耗时短、 设备操作简便, 为市场大规模的快速检测提供了现场、 高效、 灵敏的解决方案。
Abstract
New method for rapid and quantitative analysis of 6-benzylaminopurine (6-BA) residues in sprout beans was studied by using FAST-SPE and portable surface-enhanced Raman spectroscopy (SERS). With homemade sprout bean samples as blank control, 6-BA solutions extracted from inserted-treatment samples showed typical characteristic Raman peaks at 1 002 cm-1 tested by SERS, and normalized 1 002 cm-1 intensities had a good linear relationship with gradient concentrations of 6-BA insert-standard solutions. The high concentration linear range was 0.5~14 μg·mL-1, and the low one was 0.1~2 μg·mL-1. The quantitative detection limit was down to 0.02 mg·kg-1 that was below the maximum allowable residues (MAL) of 0.2 mg·kg-1 in related National Standard. The recoveries were 82.3%~95.1% and the RSD was below 5%. This method with portable equipment and simple pre-treatment showed good reproducibility, short test time-consuming and easy operating, and thus it would provide efficient and sensitive solutions for large-scale on-site and rapid detections.

张萍, 郑大威, 刘晶, 钟儒刚, 刘春伟, 郭浔, 汪泓. 基于表面增强拉曼光谱技术的豆芽6-BA残留快速检测方法[J]. 光谱学与光谱分析, 2012, 32(5): 1266. ZHANG Ping, ZHENG Da-wei, LIU Jing, ZHONG Ru-gang, LIU Chun-wei, GUO Xun, WANG Hong. Rapid Detection of 6-Benzylaminopurine Residues in Sprout Beans by Surface-Enhanced Raman Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2012, 32(5): 1266.

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