以壳聚糖包覆的CdTe量子点为荧光探针， 基于荧光猝灭法建立了吉米沙星定量测定方法。 结果表明， 体系的荧光强度与吉米沙星浓度在3.46×10－9～3.46×10－7 g·L-1范围内呈良好的线性关系(r=0.999　2)， 线性回归方程为F0/F=1.063　7+0.016　7c(g·L-1)。 对2.77×10－7 g·L-1吉米沙星溶液进行7次平行测定的相对标准偏差为2.7%，基于荧光猝灭法相关理论证明了该相互作用过程为静态猝灭。 本方法灵敏度高， 检测线性范围宽， 为吉米沙星定量测定提供了简便可靠的方法。

Chitosan-coated CdTe quantum dots can reduce QDs toxicity and enhance its stability in aqueous solution. Chitosan-coated CdTe QDs were used as fluorescence probes to determine Gemifloxacin on the basis of fluorescence quenching method. The results indicated that the relative fluorescence intensity was linearly proportional to the Gemifloxacin concentration in the range of 3.46×10－9～3.46×10－7 g·L-1 with a linear fitting equation of F0/F=1.0637+0.016 7c(g·L-1) and the RSD was 2.7%. On the basis of fluorescence quenching method theory, it was concluded that the interaction between QDs and Gemifloxacin was a kind of static quenching through hydrogen bonding and Van der Waals force, and the binding sites value was 0.8. This method with high sensitivity and broad linear range provided a new approach to determining Gemifloxacin.