光谱学与光谱分析, 2012, 32 (12): 3193, 网络出版: 2013-01-14
傅里叶变换红外光谱对枯草芽孢杆菌的光学特性研究
Optical Properties Research of Bacillus Subtilis Spores by Fourier Transform Infrared Spectroscopy
摘要
使用傅里叶变换红外光谱(FTIR)技术, 测量了两种不同浓度的枯草芽孢杆菌的红外透过率谱, 根据朗伯-比尔定律计算出它们的质量消光截面, 通过算出复折射率的虚部, 再使用KK(Kramers-Kronig)关系, 导出复折射率的实部, 并对实验结果作了分析和讨论。 通过研究枯草芽孢杆菌复折射率的测量和分析方法, 对于进一步研究生物气溶胶的吸收和散射特性、 拓宽生物气溶胶的测量和遥测技术方法, 具有重要的意义。
Abstract
The authors measured IR transmission spectra of two different concentrations of bacillus subtilis spores by using Fourier transform infrared spectroscopy (FTIR) technology. The mass extinction cross section k of bacillus subtilis spores was calculated according to Lambert-Beer law and the imaginary part ni of the complex refractive index was also calculated through k. The real part nr of the complex refractive index was derived from the KK (Kramers-Kronig) relationship and the experimental results were also analyzed and discussed with the study of measurement and analysis method of the complex refractive index on bacillus subtilis spores, it is of great significance to further research the absorption and scattering characteristics, and to broaden the measurement and remote sensing technology method of the biological aerosols.
冯明春, 徐亮, 高闽光, 焦洋, 魏秀丽, 金岭, 程巳阳, 李相贤, 冯书香. 傅里叶变换红外光谱对枯草芽孢杆菌的光学特性研究[J]. 光谱学与光谱分析, 2012, 32(12): 3193. FENG Ming-chun, XU Liang, GAO Min-guang, JIAO Yang, WEI Xiu-li, JIN Ling, CHENG Si-yang, LI Xiang-xian, FENG Shu-xiang. Optical Properties Research of Bacillus Subtilis Spores by Fourier Transform Infrared Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2012, 32(12): 3193.