运用傅里叶红外光谱、 三维荧光光谱、 透射电镜等技术, 研究了溶藻菌株Streptomyces sp. HJC-D1抑制铜绿微囊藻过程中溶解性有机物(DOM)的组成与特性, 初步揭示了微生物溶藻机理。 结果表明, 溶藻菌株发酵液添加量5%(φ)时铜绿微囊藻去除率可达72.6%±5.5%; 溶藻产物DOM以类腐殖酸物质为主, 平均分子量在1 000 Da左右; 溶藻过程藻细胞结构遭到明显破坏, 推测可能的微生物溶藻机理为: 溶藻菌破坏铜绿微囊藻细胞壁, 导致藻细胞失活、 死亡。

Using 3-D fluorescence spectroscopy (EEMs), infrared spectroscopy (FTIR) and transmission electron microscope (TEM), the composition and characteristics of dissolved organic matter (DOM) from degradation products and inhibition mechanism of Streptomyces sp. HJC-D1 on Microcystis aeruginosa were studied. The results indicated that the growth of M. aeruginosa could be effectively inhibited by the fermentation broth of HJC-D1 and the removal efficiency was 72.6%±5.5% with an addition dosage of 5% (φ). The main fluorescent material in DOM was humic-like acid, and the molecular weight of degradation products was around 1 000 Da. The cell structure of M. aeruginosa was damaged during the biodegradation process. With the results of TEM, the antialgal mechanism was speculated as following: M. aeruginosa cell walls are destroyed by antialgal bacterium, and organelles are released which resulted in the death of algae cell finally.