根据有机物受激发射荧光的基本原理, 研究了杀虫剂吡虫啉的荧光特性。 利用荧光光谱仪对吡虫啉及苹果汁-吡虫啉混合体系进行了研究, 得到了苹果汁中不同农药含量下的荧光谱图, 分析了荧光强度与农药含量的关系。 实验表明: 当234 nm作为激发波长时, 苹果果汁几乎没有荧光峰出现, 而吡虫啉有很强的荧光, 荧光峰值在373 nm左右。 向果汁中逐量添加吡虫啉药液后, 得到苹果汁中吡虫啉含量最佳预测模型, 决定系数达到0.996 74, 预测准确率高于90%。 研究结果表明, 利用荧光光谱法直接对苹果果汁中农药残留检测是快速和可行有效的。

The fluorescence properties of imidacloprid was studied based on the basic theory that organic molecules can emit fluorescence as they are excited by rays. The fluorescence spectra were obtained under the condition of different content of imidacloprid in apple juices and pure apple juices respectively through fluorescence spectrometer, and the relation between their fluorescence intensity and content of imidacloprid was analyzed. The experiment results show that the most intensive fluorescence (373 nm) was found in the spectrum of imidacloprid, while the fluorescence was not found in the pure apple juices with 234 nm as the excitation wavelength. Then the imidacloprid solution was added to the fruit juices increasingly. The best prediction model was obtained for the contend of imidacloprid in the apple juices, the coefficient of determination is 0.996 74, and the accuracy is higher than 90%. As a result, it is fast and feasible to carry out the detection and analysis of the pesticide residue of imidacloprid in the apple juices.