光学学报, 2013, 33 (9): 0918001, 网络出版: 2013-07-16
微腔光声探测技术及其成像方法
Microcavity Photoacoustic Detecting Technology and Imaging Method
摘要
提出了具有高探测灵敏度的微腔光声探测技术。采用微腔耦合技术,将细胞内产生的光声信号,通过微通道传导到耦合腔,被麦克风接收,成功研制出微腔光声探测器。在相同的温度变化情况下,密闭腔内气压变化量与腔的体积成反比,腔的体积越小,气压变化量越大,探测器对压力的响应度就越高,对于微弱光声信号就越灵敏。因此,设计出的高灵敏度微腔光声探测器,能够探测到连续调制光激发出的微弱光声信号,重建出免标记的细胞水平的光声显微图像。从已获得的分辨率板、洋葱表皮细胞和血红细胞等样品的光声显微图像可以说明这个光声微腔探测器的灵敏度高,分辨率达到1.25 μm。
Abstract
Employing microcavity coupling technology, a microcavity photoacoustic transducer with high sensitivity is proposed. The transducer works like this: the photoacoustic (PA) waves, which carry the information of the sample, transmit across the microchannel to the coupling cavity and are detected by the microphone. In the same case of the temperature change, the pressure variation in an airtight cavity is inversely proportional to the volume of the cavity. So the sensitivity of transducer can be effectively increased by using the microcavity structure, which is more sensitive to the feeble PA signal induced by a modulated continuous wave laser source. By using the technology, the photoacoustic microscopy (PAM) images of onion epidermal cells and RBCs are clearly reconstructed in cellular resolution. The result of resolution test target verifies the resolution of the PAM with microcavity PA transducer is better than 1.25 μm.
蔡秋霞, 唐志列, 吴泳波, 谭治良, 张曼. 微腔光声探测技术及其成像方法[J]. 光学学报, 2013, 33(9): 0918001. Cai Qiuxia, Tang Zhilie, Wu Yongbo, Tan Zhiliang, Zhang Man. Microcavity Photoacoustic Detecting Technology and Imaging Method[J]. Acta Optica Sinica, 2013, 33(9): 0918001.