理论模拟与分析了皮肤基底细胞癌症组织的自体荧光光谱特征.在实验分析基底细胞癌症组织显微荧光图像特征基础上, 建立相应的八层组织光学模型.采用蒙特卡罗模拟方法, 分析基底细胞癌症组织在可见光波段的漫反射特征, 并对光学模型进行完善.同时, 基于光与组织的相互作用机理与蒙特卡罗方法, 模拟分析皮肤组织结构对其自体荧光光子的吸收和散射作用, 讨论自体荧光在组织内部的传输机制, 并最终重构在体基底细胞癌症组织的荧光光谱.结果表明, 理论重构的荧光光谱与在体实验结果基本一致.本研究工作为分析研究癌症病变组织内的光谱特性, 阐述生物组织内部自体荧光产生与传播机制, 提供了一种理论研究方法.

Autofluorescence characteristics of Basal Cell Carcinoma skin were studied by Monte Carlo method. Based on the microscopic autofluorescence image, an eight-layered optical model of skin Basal Cell Carcinoma was constructed. By Monte Carlo simulation, the light distribution in the model was calculated, and the diffuse reflectance process was modeled in visible wavelength range in order to perfect the multi-layer tissues optics model. Monte Carlo method was used to simulate the tissues reabsorbtion and rescattering effect on intrinsic autofluorescence based on the light-tissue interactions. The escaping process of autofluorescence photons from tissue surface was discussed, and the in vivo autofluoresconce spectra of skin Basal Cell Carcinoma were reconstructed. The results show that the rebuilted skin autofluorescence spectra achieve a good agreement with the experimental results. This study will provide a practical theoretical method to study transmission process of intrinsic autofluorescence in skin cancer lesions, and revealed autofluorescence characteristics of skin cancer.